MiR-495-3p promotes cardiac hypertrophy by targeting Pum2.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-09-08 DOI:10.14715/cmb/2024.70.8.15

Shushu Yu, Mingliang Wang, Yun Xie, Bo Wang, Yawei Xu

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引用次数: 0

Abstract

Pathological cardiac hypertrophy (CH) may lead to heart failure and sudden death. MicroRNAs (miRNAs) have been documented to play crucial parts in CH. The objective of this research was to discuss the potential along with molecule mechanism of miR-495-3p in CH. In vivo CH model was induced by aortic banding (AB) in rats. Cellular hypertrophy in H9c2 rat cardiomyocytes was stimulated by angiotensin II (Ang II) treatment. Haematoxylin and eosin (HE), echocardiography and immunofluorescence staining were used to examine the alterations in cardiac function. The outcomes showed that miR-495-3p expression was high in rat model as well as in Ang II-stimulated cardiomyocytes. Besides, silenced miR-495-3p attenuated CH both in vitro and in vivo. Mechanically, miR-495-3p bound to pumilio RNA binding family member 2 (Pum2) 3'UTR and silenced its expression. Rescue assays further notarized that Pum2 silence abrogated the inhibitory impacts of miR-495-3p inhibitor on CH. In a word, the present research uncovered that miR-495-3p promoted CH by targeting Pum2. Therefore, miR-495-3p may be a novel therapeutic molecule for this disease.

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MiR-495-3p 通过靶向 Pum2 促进心脏肥大

病理性心脏肥大（CH）可能导致心力衰竭和猝死。有文献表明，微RNA（miRNA）在心肌肥大中起着至关重要的作用。本研究旨在探讨 miR-495-3p 在 CH 中的潜在作用和分子机制。大鼠主动脉束带（AB）诱导了体内CH模型。血管紧张素II（Ang II）刺激H9c2大鼠心肌细胞肥大。研究采用了血栓素和伊红（HE）、超声心动图和免疫荧光染色来检测心脏功能的改变。结果表明，miR-495-3p 在大鼠模型和 Ang II 刺激的心肌细胞中均有高表达。此外，沉默的 miR-495-3p 在体外和体内均可减轻 CH。在机制上，miR-495-3p 与 pumilio RNA 结合家族成员 2（Pum2）3'UTR 结合并抑制其表达。拯救实验进一步证实，Pum2 的沉默会减弱 miR-495-3p 抑制剂对 CH 的抑制作用。总之，本研究发现，miR-495-3p 通过靶向 Pum2 促进了 CH。因此，miR-495-3p可能是治疗这种疾病的新型分子。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.