Homologous mapping yielded a comprehensive predicted protein-protein interaction network for peanut (Arachis hypogaea L.).

IF 5.4 3区材料科学 Q2 CHEMISTRY, PHYSICAL

ACS Applied Energy Materials Pub Date : 2024-09-20 DOI:10.1186/s12870-024-05580-w

Fangping Gong, Di Cao, Xiaojian Sun, Zhuo Li, Chengxin Qu, Yi Fan, Zenghui Cao, Kai Zhao, Kunkun Zhao, Ding Qiu, Zhongfeng Li, Rui Ren, Xingli Ma, Xingguo Zhang, Dongmei Yin

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引用次数: 0

Abstract

Background: Protein-protein interactions are the primary means through which proteins carry out their functions. These interactions thus have crucial roles in life activities. The wide availability of fully sequenced animal and plant genomes has facilitated establishment of relatively complete global protein interaction networks for some model species. The genomes of cultivated and wild peanut (Arachis hypogaea L.) have also been sequenced, but the functions of most of the encoded proteins remain unclear.

Results: We here used homologous mapping of validated protein interaction data from model species to generate complete peanut protein interaction networks for A. hypogaea cv. 'Tifrunner' (282,619 pairs), A. hypogaea cv. 'Shitouqi' (256,441 pairs), A. monticola (440,470 pairs), A. duranensis (136,363 pairs), and A. ipaensis (172,813 pairs). A detailed analysis was conducted for a putative disease-resistance subnetwork in the Tifrunner network to identify candidate genes and validate functional interactions. The network suggested that DX2UEH and its interacting partners may participate in peanut resistance to bacterial wilt; this was preliminarily validated with overexpression experiments in peanut.

Conclusion: Our results provide valuable new information for future analyses of gene and protein functions and regulatory networks in peanut.

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同源映射产生了一个全面的花生（Arachis hypogaea L.）预测蛋白质-蛋白质相互作用网络。

背景：蛋白质与蛋白质之间的相互作用是蛋白质发挥其功能的主要途径。因此，这些相互作用在生命活动中起着至关重要的作用。全序列动物和植物基因组的广泛存在，为一些模式物种建立相对完整的全球蛋白质相互作用网络提供了便利。栽培和野生花生（Arachis hypogaea L.）的基因组也已测序，但大多数编码蛋白的功能仍不清楚：结果：我们利用同源映射模型物种的有效蛋白质相互作用数据，为A. hypogaea cv. 'Tifrunner'（282,619 对）、A. hypogaea cv. 'Shitouqi'（256,441 对）、A. monticola（440,470 对）、A. duranensis（136,363 对）和 A. ipaensis（172,813 对）生成了完整的花生蛋白质相互作用网络。我们对 Tifrunner 网络中一个假定的抗病性子网络进行了详细分析，以确定候选基因并验证功能相互作用。该网络表明，DX2UEH 及其相互作用伙伴可能参与了花生对细菌性枯萎病的抗性；在花生中进行的过表达实验初步验证了这一点：我们的研究结果为今后分析花生基因和蛋白质功能及调控网络提供了宝贵的新信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Energy Materials Materials Science-Materials Chemistry

CiteScore

10.30

自引率

6.20%

发文量

1368

期刊介绍： ACS Applied Energy Materials is an interdisciplinary journal publishing original research covering all aspects of materials, engineering, chemistry, physics and biology relevant to energy conversion and storage. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important energy applications.