Pumpkin seed oil: unveiling its potential in controlling inflammation and pathogenicity during experimental trichinellosis.

IF 2.3 2区农林科学 Q1 VETERINARY SCIENCES

BMC Veterinary Research Pub Date : 2024-09-20 DOI:10.1186/s12917-024-04241-2

Sara Salah Abdel-Hakeem, Sulaiman Mohammed Alnasser, Alotaibi Meshal, Mahmoud Abdel-Zaher Abdel-Samiee, Mohamed Salah Eldin Youssef, Shimaa Hamdi Abd Elsadek, Salwa Mahmoud Abd-Elrahman

{"title":"Pumpkin seed oil: unveiling its potential in controlling inflammation and pathogenicity during experimental trichinellosis.","authors":"Sara Salah Abdel-Hakeem, Sulaiman Mohammed Alnasser, Alotaibi Meshal, Mahmoud Abdel-Zaher Abdel-Samiee, Mohamed Salah Eldin Youssef, Shimaa Hamdi Abd Elsadek, Salwa Mahmoud Abd-Elrahman","doi":"10.1186/s12917-024-04241-2","DOIUrl":null,"url":null,"abstract":"Background: This study aimed to investigate the antiparasitic and anti-inflammatory potential of pumpkin seed oil in mice infected with Trichinella spiralis by demonstrating its impact on MMP-9 expression and pathogenesis during the intestinal and muscular phases.Results: In this study, 100 mice were divided into five groups: an infected group, a pumpkin seed oil-treated group (1.5 mg/kg BW, administered three times per week), an albendazole-treated group, a native control group, and a pumpkin oil control group. Gas chromatography-mass spectrometry analysis of the pumpkin seed oil revealed a broad spectrum of biologically active compounds. The pumpkin seed oil treatment led to a significant reduction in the parasite burden, with a 75% decrease in adult worms and a 66% decrease in encysted larvae. Additionally, the infected animals treated with pumpkin oil exhibited a marked reduction in intestinal inflammation, characterized by a progressive increase in goblet cells. The number of encysted larvae in the diaphragm and muscle tissues was also significantly decreased. Furthermore, pumpkin seed oil treatment significantly reduced MMP-9 levels in both intestinal and muscular tissues, highlighting its potential to attenuate inflammation.Conclusion: These findings underscore the effectiveness of pumpkin seed oil as anti-inflammatory and antiparasitic agent.","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":null,"pages":null},"PeriodicalIF":2.3000,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11414094/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"BMC Veterinary Research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1186/s12917-024-04241-2","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Background: This study aimed to investigate the antiparasitic and anti-inflammatory potential of pumpkin seed oil in mice infected with Trichinella spiralis by demonstrating its impact on MMP-9 expression and pathogenesis during the intestinal and muscular phases.

Results: In this study, 100 mice were divided into five groups: an infected group, a pumpkin seed oil-treated group (1.5 mg/kg BW, administered three times per week), an albendazole-treated group, a native control group, and a pumpkin oil control group. Gas chromatography-mass spectrometry analysis of the pumpkin seed oil revealed a broad spectrum of biologically active compounds. The pumpkin seed oil treatment led to a significant reduction in the parasite burden, with a 75% decrease in adult worms and a 66% decrease in encysted larvae. Additionally, the infected animals treated with pumpkin oil exhibited a marked reduction in intestinal inflammation, characterized by a progressive increase in goblet cells. The number of encysted larvae in the diaphragm and muscle tissues was also significantly decreased. Furthermore, pumpkin seed oil treatment significantly reduced MMP-9 levels in both intestinal and muscular tissues, highlighting its potential to attenuate inflammation.

Conclusion: These findings underscore the effectiveness of pumpkin seed oil as anti-inflammatory and antiparasitic agent.

查看原文本刊更多论文

南瓜籽油：揭示其在实验性毛霉菌病中控制炎症和致病性的潜力。

背景：本研究旨在通过证明南瓜籽油对MMP-9表达的影响以及在肠道和肌肉阶段的致病机理，探讨南瓜籽油在小鼠螺旋毛霉感染中的抗寄生虫和抗炎潜力：本研究将 100 只小鼠分为五组：感染组、南瓜籽油处理组（1.5 毫克/千克体重，每周给药三次）、阿苯达唑处理组、本地对照组和南瓜籽油对照组。对南瓜籽油进行的气相色谱-质谱分析表明，其中含有多种生物活性化合物。经南瓜籽油处理后，寄生虫的数量显著减少，成虫减少了 75%，包囊幼虫减少了 66%。此外，用南瓜籽油处理的受感染动物的肠道炎症明显减轻，其特征是鹅口疮细胞逐渐增多。膈肌和肌肉组织中包囊幼虫的数量也明显减少。此外，南瓜籽油还能明显降低肠道和肌肉组织中的 MMP-9 水平，突出了其减轻炎症的潜力：这些发现强调了南瓜籽油作为抗炎和抗寄生虫药物的有效性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

BMC Veterinary Research VETERINARY SCIENCES-

CiteScore

4.80

自引率

3.80%

发文量

420

审稿时长

3-6 weeks

期刊介绍： BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.