Correlation between Interleukin-10 Effects on Th1/Th2 Immune Balance and Pregnancy Induced Hypertension.

IF 1.1 4区医学 Q4 MEDICAL LABORATORY TECHNOLOGY

Annals of clinical and laboratory science Pub Date : 2024-07-01

Yu Shi, Bing Han, Xianchi Li, Xiaojing Yuan

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引用次数: 0

Abstract

Objective: Pregnancy induced hypertension (PIH) is a common disease in obstetrics. CD4+ T cells can be divided into Th1 and Th2 sub-populations. Imbalance between Th1 and Th2 directly affects body immune status and participates in PIH occurrence and progression. Whether IL-10 affects Th1/Th2 immune balance as a negative regulator of immune response in PIH remains unknown. The aim of the present study was to investigate the role of IL-10 in PIH.

Methods: A total of 52 PIH patients were recruited and divided into mild-moderate and severe PIH groups in parallel with 25 normal pregnant women as a control group. Real-time PCR was used to test mRNA levels of Th1 cytokines IL-2, tumor necrosis factor-α (TNF-α), and Th2 cytokines IL-4, IL-6, and IL-10. Enzyme linked immunosorbent assay (ELISA) tested serum levels of cytokines to analyze their correlation with disease progression.

Results: Our results showed PIH patients had significantly elevated IL-2 and TNF-α levels and decreased IL-4, IL-6, or IL-10 expressions compared with the control group (p<0.05). With disease progression, IL-4, IL-6, and IL-10 expressions were further decreased while IL-2 and TNF-α were increased (p<0.05). Moreover, IL-10 was negatively correlated with Th1 cytokines IL-2 and TNF-α while being positively correlated with Th2 cytokines IL-4 and IL-6. In addition, IL-10 was negatively correlated with PIH severity (p<0.05).

Conclusion: IL-10 can affect Th1/Th2 immune balance and is associated with PIH severity, suggesting IL-10 might be a risk factor for PIH occurrence and progression.

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白细胞介素-10 对 Th1/Th2 免疫平衡的影响与妊娠诱发高血压之间的相关性

目的：妊娠高血压（PIH）是产科常见疾病。CD4+ T 细胞可分为 Th1 和 Th2 亚群。Th1 和 Th2 的失衡直接影响机体免疫状态，并参与 PIH 的发生和发展。作为 PIH 免疫应答的负调控因子，IL-10 是否会影响 Th1/Th2 免疫平衡仍是未知数。本研究旨在探讨 IL-10 在 PIH 中的作用：方法：共招募了 52 名 PIH 患者，将其分为轻中度和重度 PIH 组，并同时招募 25 名正常孕妇作为对照组。采用实时 PCR 检测 Th1 细胞因子 IL-2、肿瘤坏死因子-α（TNF-α）和 Th2 细胞因子 IL-4、IL-6 和 IL-10 的 mRNA 水平。酶联免疫吸附试验（ELISA）检测血清中细胞因子的水平，分析它们与疾病进展的相关性：结果显示，与对照组相比，PIH 患者的 IL-2 和 TNF-α 水平明显升高，而 IL-4、IL-6 或 IL-10 的表达则明显降低（pα 升高），同时与 Th2 细胞因子 IL-4 和 IL-6 呈正相关。此外，IL-10 与 PIH 严重程度呈负相关（pConclusion）：IL-10可影响Th1/Th2免疫平衡，并与PIH严重程度相关，这表明IL-10可能是PIH发生和发展的一个危险因素。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Annals of clinical and laboratory science 医学-医学实验技术

CiteScore

1.60

自引率

0.00%

发文量

112

审稿时长

6-12 weeks

期刊介绍： The Annals of Clinical & Laboratory Science welcomes manuscripts that report research in clinical science, including pathology, clinical chemistry, biotechnology, molecular biology, cytogenetics, microbiology, immunology, hematology, transfusion medicine, organ and tissue transplantation, therapeutics, toxicology, and clinical informatics.