Validation of monocyte CD169 expression as a valuable rapid diagnostic marker of SARS-CoV-2 and other acute viral infections.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2024-09-21 DOI:10.1093/ajcp/aqae127

Chiara Pratesi, Rita De Rosa, Eliana Pivetta, Kathreena Vattamattathil, Giacomo Malipiero, Desré Ethel Fontana, Giancarlo Basaglia, Paolo Doretto

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引用次数: 0

Abstract

Objectives: Acute infectious diseases are some of the most common reasons for receiving medical care, and analysis of the host immune response is an attractive approach for their diagnosis. The present study aimed to evaluate the potential usefulness of CD169 expression on peripheral monocytes (mCD169) as a marker of viral-associated host immune response.

Methods: In a large mono-institutional cohort of 4,025 patients evaluated for SARS-CoV-2 (CoV2) and other viral infections, mCD169 analysis was performed by rapid flow cytometry assay.

Results: Increased mCD169 values (median, 17.50; IQR, 8.40-25.72) were found in 1,631 patients with CoV2+ acute infection compared to 2,394 in CoV2- patients (median, 2.35; IQR, 2.0-3.25) (odds ratio [OR], 21.84; 95% CI ,17.53-27.21; P < .001). Among CoV2- patients, 1,484 (62.0%) were assessed for other viral infections, and viral etiology was laboratory confirmed in 428 patients (CoV2- Vir+), with RNA viruses most frequently detected (94.6%). Higher levels of mCD169 were also confirmed in CoV2- Vir+ compared to CoV2- Vir- patients (OR, 10.05; 95% CI, 7.35-13.74; P < .001).

Conclusions: mCD169 analysis by rapid flow cytometry assay may be a sensitive broad marker useful for the rapid triage of patients with suspected acute viral infections and could potentially be directly applied to eventual new emergent viral outbreaks.

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验证单核细胞 CD169 表达是 SARS-CoV-2 和其他急性病毒感染的重要快速诊断标志物。

目的：急性传染病是一些最常见的就医原因，而分析宿主免疫反应是诊断急性传染病的一种有吸引力的方法。本研究旨在评估外周单核细胞上 CD169 表达（mCD169）作为病毒相关宿主免疫反应标志物的潜在作用：方法：在一个由 4,025 名 SARS-CoV-2（CoV2）和其他病毒感染患者组成的大型单一机构队列中，采用快速流式细胞术检测法对 mCD169 进行分析：在 1631 名 CoV2+ 急性感染患者中发现 mCD169 值升高（中位数，17.50；IQR，8.40-25.72），而在 2394 名 CoV2 患者中发现 mCD169 值升高（中位数，2.35；IQR，2.0-3.25）（几率比 [OR]，21.84；95% CI ,17.53-27.21；P < .001）。在 CoV2- 患者中，1,484 例（62.0%）患者接受了其他病毒感染评估，428 例患者（CoV2- Vir+）的病毒病因得到实验室证实，其中最常检测到的是 RNA 病毒（94.6%）。结论：通过快速流式细胞术分析 mCD169 可能是一种灵敏的广泛标记物，可用于快速分流疑似急性病毒感染患者，并有可能直接应用于最终的新突发病毒疫情。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464