Paper-based multicolor sensor for on-site quantitative detection of organophosphate pesticides based on acetylcholinesterase-mediated paper-based Au³⁺-etching of gold nanobipyramids and CIELab color space.

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2025-01-01 Epub Date: 2024-09-19 DOI:10.1016/j.talanta.2024.126925

Feng Zhang, Yu Gao, Enxi Ren, Ling Fang, Weijuan Yang, Liaoyuan Zhang, Zongwen Wang

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引用次数: 0

Abstract

On-site quantitative detection of organophosphorus pesticides (OPs) is crucial for safeguarding food and public safety. This study presents a novel acetylcholinesterase (AChE)-mediated paper-based Au³⁺-etching of gold nanobipyramids (AuNBPs) system. The system employs a long-term storable AuNBPs-deposited nylon membrane embedded within a portable and temperature-controlled paper-based analytical device. This system, coupled with a colorimeter-based quantitative method, enables the development of a practical paper-based multicolor sensor (PMS) for on-site quantitative detection of three common OPs (paraoxon, dichlorvos, and trichlorfon). In the absence of OPs, AChE hydrolyzes acetylthiocholine to thiocholine, which reduces Au³⁺ to Au⁺. The presence of OPs inhibits AChE activity, thereby preserving Au³⁺ to etch AuNBPs on nylon membranes, accompanied by multicolor changes. These color changes can be simply quantified by measuring the a∗ parameter of the CIELab color space using a portable colorimeter. Under optimal conditions, the PMS displayed eight OPs-corresponding color changes with a minimum detectable concentration of 1.0-10 μg/L (visual observation) and limits of detection of 0.8-7.2 μg/L (colorimeter) and 0.2-3.4 μg/L (UV-vis spectrometry). The PMS successfully determined the OPs in vegetable and rice samples with recoveries of 89.0-109 % and RSDs (n = 5) of <6 %. These results were consistent with those obtained using the HPLC-MS method. The PMS demonstrates excellent portability, AuNBPs stability, detection sensitivity, and reproducibility, making it a promising tool for the on-site quantitative detection of OPs residues in food. Furthermore, the paper-based etching system coupled with the colorimeter-based quantitative method provides a valuable reference to develop practical PMSs for various targets in diverse fields.

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基于乙酰胆碱酯酶介导的纸基 Au3+-蚀刻金纳米双锥体和 CIELab 色彩空间的有机磷农药现场定量检测纸基多色传感器。

现场定量检测有机磷农药（OPs）对保障食品和公共安全至关重要。本研究提出了一种新型乙酰胆碱酯酶（AChE）介导的纸基 Au3+ 蚀刻金纳米双金字塔（AuNBPs）系统。该系统采用了一种可长期保存的 AuNBPs 沉积尼龙膜，嵌入到一个便携式温控纸基分析装置中。该系统与基于色度计的定量方法相结合，开发出一种实用的纸质多色传感器（PMS），用于现场定量检测三种常见的 OPs（对硫磷、敌敌畏和敌百虫）。在没有 OPs 的情况下，AChE 会将乙酰硫代胆碱水解为硫代胆碱，从而将 Au3+ 还原为 Au+。OPs 的存在抑制了 AChE 的活性，从而保留了 Au3+，使其能够蚀刻尼龙膜上的 AuNBPs，并伴随着多种颜色的变化。这些颜色变化可通过使用便携式色度计测量 CIELab 色彩空间的 a∗ 参数进行简单量化。在最佳条件下，PMS 显示了八种 OPs 对应的颜色变化，最低检测浓度为 1.0-10 μg/L（目测），检测限为 0.8-7.2 μg/L（色度计）和 0.2-3.4 μg/L（紫外-可见光谱法）。PMS 成功地测定了蔬菜和大米样品中的 OPs，回收率为 89.0-109%，RSD（n = 5）为

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.