Complex influence of structural characteristics of low-methoxyl pectins on rheological properties of semi-solid Ca-gels

IF 7.7 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules Pub Date : 2024-09-13 DOI:10.1016/j.ijbiomac.2024.135633

Olga A. Patova, Nadegda V. Kosolapova, Victoria V. Golovchenko

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引用次数: 0

Abstract

The semi-solid Ca-gels were prepared from low-methoxyl pectins (methoxylation degree DM 4–36 %) of Oberna behen, Potamogeton natans, Tanacetum vulgare, Comarum palustre, Bergenia crassifolia, and Heracleum sosnowskyi. Pectins are characterized by Mw of 77–556 kDa and different content of HG and RG-I. The gels were prepared by addition of Ca²⁺ ions at varying stoichiometric ratios, R = 2 × [Ca²⁺]/[COO⁻], of 0.2–0.6, and a fixed pectin concentration of 10 g/L. It was shown that the presence of certain sets of structural characteristics in low-methoxyl pectins from different sources leads to the formation of gels with similar rheological properties. Pectins with Mw 77–98 kDa, DM 6–14 % form stiffer Ca-gels than pectins with Mw 218–253 kDa, DM 24–36 % at the same R. Pectins with Mw 346–556 kDa form the strongest Ca-gels. Their high Mw compensates for the influence of DM on the properties of Ca-gels. The stability of gels in PBS (pH 7.4) is determined mainly by proportion of 1,4-linked non-methoxylated GalA in pectins. Pectins with DM 4–14 % form more stable Ca-gels in PBS than pectins with DM 21–36 %.

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低甲氧基果胶结构特征对半固态钙凝胶流变特性的复杂影响

用 Oberna Behen、Potamogeton natans、Tanacеtum vulgаre、Comarum palustre、Bergenia crassifolia 和 Heracleum sosnowskyi 的低甲氧基果胶（甲氧基化程度 DM 4-36%）制备了半固体钙凝胶。果胶的特征是分子量为 77-556 kDa，HG 和 RG-I 含量不同。凝胶是在果胶浓度为 10 克/升的固定条件下，以 0.2-0.6 的不同化学计量比 R = 2 × [Ca2+]/[COO-] 加入 Ca2+ 离子制备的。研究表明，不同来源的低甲氧基果胶具有某些结构特征，会形成具有相似流变特性的凝胶。在相同的 R 条件下，Mw 77-98 kDa、DM 6-14 % 的果胶比 Mw 218-253 kDa、DM 24-36 % 的果胶形成的钙凝胶更硬。它们的高分子量弥补了 DM 对 Ca 凝胶特性的影响。凝胶在 PBS（pH 值为 7.4）中的稳定性主要取决于果胶中 1,4 连接的非甲氧基化 GalA 的比例。在 PBS 中，DM 值为 4-14% 的果胶比 DM 值为 21-36% 的果胶形成的钙凝胶更稳定。

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来源期刊

International Journal of Biological Macromolecules 生物-生化与分子生物学

CiteScore

13.70

自引率

9.80%

发文量

2728

审稿时长

64 days

期刊介绍： The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.