Direct and indirect effects of chemical pollution: Fungicides alter growth, feeding, and pigmentation of the freshwater detritivore Asellus aquaticus

Abstract

Anthropogenic chemical pollutants, such as fungicides, pose significant threats to natural ecosystems. Although the direct impacts of numerous chemicals are well-documented in simple environmental contexts, their indirect impacts are poorly understood. This study used two individual level laboratory experiments to assess direct and indirect effects of fungicides on the isopod Asellus aquaticus, a keystone detritivore in freshwater systems. First, a range-finding assay on three widely used fungicides (Fluazinam, Tebuconazole, Urea) showed that Tebuconazole had the strongest concentration-dependent negative effects on A. aquaticus growth and food consumption. Second, a factorial experiment using Tebuconazole assessed its direct and diet-mediated effects and showed that Tebuconazole reduced growth, feeding, and pigmentation through both pathways. The results indicate that assessing only direct impacts of toxic chemicals could overlook critical interactions that are relevant in natural systems, such as those associated with diet. Our study highlights the importance of considering both direct and indirect effects in environmental toxicology to better understand the full impacts of chemical pollutants in nature.