A disposable fiber-optic plasmonic sensor for chemical sensing

IF 2.6 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Analytical biochemistry Pub Date : 2024-09-16 DOI:10.1016/j.ab.2024.115672

引用次数: 0

Abstract

The integration of fiber optics and plasmonic sensors is promising to improve the practical usability over conventional bulky sensors and systems. To achieve high sensitivity, it typically requires fabrication of well-defined plasmonic nanostructures on optical fibers, which greatly increases the cost and complexity of the sensors. Here, we present a fiber-optic sensor system by using chemical absorption of gold nanoparticles and a replaceable configuration. By functioning gold nanoparticles with aptamers or antibodies, we demonstrate the applications in chemical sensing using two different modes. Measuring shift in resonance wavelength enables the Pb²⁺ detection with a high linearity and a limit of detection of 0.097 nM, and measuring absorption peak amplitude enables the detection of E. coli in urinary tract infection with a dynamic range between 10³ to 10⁸ CFU/mL. The high sensitivity, simple fabrication and disposability of this sensing approach could pave the way for point-of-care testing with fiber-optic plasmonic sensors.

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用于化学传感的一次性光纤质子传感器

与传统笨重的传感器和系统相比，光纤和等离子传感器的集成有望提高实际可用性。要实现高灵敏度，通常需要在光纤上制造定义明确的质子纳米结构，这大大增加了传感器的成本和复杂性。在这里，我们提出了一种利用金纳米粒子的化学吸收和可更换配置的光纤传感器系统。通过金纳米粒子与适配体或抗体的作用，我们展示了两种不同模式在化学传感中的应用。测量共振波长的偏移可实现对 Pb2+ 的检测，线性度高，检测限为 0.097 nM；测量吸收峰的振幅可实现对尿路感染中大肠杆菌的检测，动态范围为 103-108 CFU/mL。这种传感方法具有灵敏度高、制作简单和便于携带等特点，可为使用光纤质子传感器进行护理点检测铺平道路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Analytical biochemistry 生物-分析化学

CiteScore

5.70

自引率

0.00%

发文量

283

审稿时长

44 days

期刊介绍： The journal''s title Analytical Biochemistry: Methods in the Biological Sciences declares its broad scope: methods for the basic biological sciences that include biochemistry, molecular genetics, cell biology, proteomics, immunology, bioinformatics and wherever the frontiers of research take the field. The emphasis is on methods from the strictly analytical to the more preparative that would include novel approaches to protein purification as well as improvements in cell and organ culture. The actual techniques are equally inclusive ranging from aptamers to zymology. The journal has been particularly active in: -Analytical techniques for biological molecules- Aptamer selection and utilization- Biosensors- Chromatography- Cloning, sequencing and mutagenesis- Electrochemical methods- Electrophoresis- Enzyme characterization methods- Immunological approaches- Mass spectrometry of proteins and nucleic acids- Metabolomics- Nano level techniques- Optical spectroscopy in all its forms. The journal is reluctant to include most drug and strictly clinical studies as there are more suitable publication platforms for these types of papers.