Electrochemical sensor for the analysis of 5-hydroxymethylcytosine in the presence of cytosine using pencil graphite electrode

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2024-09-16 DOI:10.1016/j.ab.2024.115674

Selva Bilge , Manolya Müjgan Gürbüz , Sibel A. Ozkan , Burcu Dogan Topal

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Abstract

In recent years, important efforts have been made to elucidate the mechanisms of epigenetic regulation, and one of the most studied epigenetic modifications was DNA methylation/demethylation. In this study, the voltammetric behaviour of 5-hydroxymethylcytosine was studied in the pH range of 2.00–11.00 using pencil graphite electrodes by differential pulse and square wave voltammetry. The effect of buffer solutions, scan rate, square wave voltammetry parameters, and stripping conditions on the voltammetric responses of 5-hydroxymethylcytosine were performed. The electrochemical oxidation process of 5-hydroxymethylcytosine on the pencil graphite electrode was realized under adsorption control. In human urine, by square wave stripping voltammetry, 5-hydroxymethylcytosine was quantified in a concentration range of 1.00 × 10⁻⁵ M-2.00 × 10⁻⁴ M. The proposed method was tested in the presence of cytosine in human urine. The recovery value of 5-hydroxymethylcytosine was found to be 99.57 %.

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使用铅笔石墨电极分析存在胞嘧啶的 5-羟甲基胞嘧啶的电化学传感器

近年来，人们一直致力于阐明表观遗传调控机制，其中研究最多的表观遗传修饰之一就是 DNA 甲基化/去甲基化。本研究使用铅笔石墨电极，通过差分脉冲和方波伏安法研究了 5-羟甲基胞嘧啶在 pH 值为 2.00-11.00 范围内的伏安行为。研究了缓冲溶液、扫描速率、方波伏安参数和剥离条件对 5-羟甲基胞嘧啶伏安响应的影响。在吸附控制下实现了 5-羟甲基胞嘧啶在铅笔石墨电极上的电化学氧化过程。通过方波剥离伏安法对人体尿液中的 5-羟甲基胞嘧啶进行了定量分析，其浓度范围为 1.00 × 10-5 M-2.00 × 10-4 M。结果发现，5-羟甲基胞嘧啶的回收率为 99.57%。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464