{"title":"Enhanced enzymatic hydrolysis of corn stover by γ-valerolactone pretreatment and the characteristics of enzymatic residues","authors":"","doi":"10.1016/j.procbio.2024.09.016","DOIUrl":null,"url":null,"abstract":"<div><p>Efficient pretreatment methods are crucial for the generation of fermentable sugars from the renewable lignocellulose. In this study, γ-valerolactone (GVL), a biomass-derived green solvent, was used to fractionate corn stover (CS). The impact of different additives on the enzymatic hydrolysis and pretreatment of CS within the GVL/water system was carefully investigated. The results demonstrate that the use of H<sub>2</sub>SO<sub>4</sub>-assisted GVL/H<sub>2</sub>O (80:20, w/w) pretreatment at mild conditions successfully facilitates the depolymerization of CS, thereby improving the cellulase accessibility. Furthermore, by increasing the GVL pretreatment temperature to 160 °C with 100 mM H<sub>2</sub>SO<sub>4</sub> addition, the pretreated CS led to nearly 100 % of hydrolysis yield at 2 % (w/v) solid load. Finally, the thermogravimetric analysis (TGA) was performed to evaluate the thermal decomposition characteristics of untreated CS and enzymatic residues based on the H<sub>2</sub>SO<sub>4</sub>-assisted GVL/H<sub>2</sub>O pretreatment. The results indicate that the enzymatic residues had two depolymerization stages from 200 °C to 500 °C with a final residual mass of 22.73 %, which was higher than that of untreated CS (20.56 %). Overall, this study provides an efficient lignocellulose pretreatment for fermentable sugars production and valorization of enzymatic residues.</p></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Process Biochemistry","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1359511324003131","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Efficient pretreatment methods are crucial for the generation of fermentable sugars from the renewable lignocellulose. In this study, γ-valerolactone (GVL), a biomass-derived green solvent, was used to fractionate corn stover (CS). The impact of different additives on the enzymatic hydrolysis and pretreatment of CS within the GVL/water system was carefully investigated. The results demonstrate that the use of H2SO4-assisted GVL/H2O (80:20, w/w) pretreatment at mild conditions successfully facilitates the depolymerization of CS, thereby improving the cellulase accessibility. Furthermore, by increasing the GVL pretreatment temperature to 160 °C with 100 mM H2SO4 addition, the pretreated CS led to nearly 100 % of hydrolysis yield at 2 % (w/v) solid load. Finally, the thermogravimetric analysis (TGA) was performed to evaluate the thermal decomposition characteristics of untreated CS and enzymatic residues based on the H2SO4-assisted GVL/H2O pretreatment. The results indicate that the enzymatic residues had two depolymerization stages from 200 °C to 500 °C with a final residual mass of 22.73 %, which was higher than that of untreated CS (20.56 %). Overall, this study provides an efficient lignocellulose pretreatment for fermentable sugars production and valorization of enzymatic residues.
期刊介绍:
Process Biochemistry is an application-orientated research journal devoted to reporting advances with originality and novelty, in the science and technology of the processes involving bioactive molecules and living organisms. These processes concern the production of useful metabolites or materials, or the removal of toxic compounds using tools and methods of current biology and engineering. Its main areas of interest include novel bioprocesses and enabling technologies (such as nanobiotechnology, tissue engineering, directed evolution, metabolic engineering, systems biology, and synthetic biology) applicable in food (nutraceutical), healthcare (medical, pharmaceutical, cosmetic), energy (biofuels), environmental, and biorefinery industries and their underlying biological and engineering principles.