{"title":"Synergistic effects of Paenibacillus mucilaginosus and Penicillium pimiteouiense on the extraction of humic substances from lignite","authors":"","doi":"10.1016/j.procbio.2024.09.006","DOIUrl":null,"url":null,"abstract":"<div><p>Based on the common step of alkaline extraction-acid precipitation, lignite treatment techniques have been developed to improve extraction efficiency and yield of humic acid (HA). This study explored the combination of bacterium Paenibacillus mucilaginosus (P.m) LT1906 and fungus Penicillium pimiteouiense (P.p) LL2205 strains for pre- and post-treatment of lignite collected from eastern Inner Mongol to enhance HA and fulvic acid (FA) yield and plant growth-promoting activity. The lignite was subjected to P.m followed by HNO3 treatment, HNO3 followed by P.p treatment, and a combination of P.m, HNO3 and P.p treatments, then performed by alkaline extraction-acid precipitation technique. The results indicated that P.m pretreatment increased HA yield by 12.47 %, while P.p posttreatment significantly enhanced FA yield by 13.37 %. Combined bacterial and fungal treatments notably improved the total yields of HA and FA by 19.12 %. Structural analysis showed that P.m pretreatment caused the deformation on the lignite surface and the complete lignite oxidation and leaching reactions, thereby increasing the production of HA, yet had no impact on the elemental composition or chemical structure of HA. P.p posttreatment introduced oxygencontaining functional groups and reduced aromatic components through oxidative depolymerization of lignite, leading to increased FA production and HA seed germination-promoting activity.</p></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Process Biochemistry","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1359511324003015","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Based on the common step of alkaline extraction-acid precipitation, lignite treatment techniques have been developed to improve extraction efficiency and yield of humic acid (HA). This study explored the combination of bacterium Paenibacillus mucilaginosus (P.m) LT1906 and fungus Penicillium pimiteouiense (P.p) LL2205 strains for pre- and post-treatment of lignite collected from eastern Inner Mongol to enhance HA and fulvic acid (FA) yield and plant growth-promoting activity. The lignite was subjected to P.m followed by HNO3 treatment, HNO3 followed by P.p treatment, and a combination of P.m, HNO3 and P.p treatments, then performed by alkaline extraction-acid precipitation technique. The results indicated that P.m pretreatment increased HA yield by 12.47 %, while P.p posttreatment significantly enhanced FA yield by 13.37 %. Combined bacterial and fungal treatments notably improved the total yields of HA and FA by 19.12 %. Structural analysis showed that P.m pretreatment caused the deformation on the lignite surface and the complete lignite oxidation and leaching reactions, thereby increasing the production of HA, yet had no impact on the elemental composition or chemical structure of HA. P.p posttreatment introduced oxygencontaining functional groups and reduced aromatic components through oxidative depolymerization of lignite, leading to increased FA production and HA seed germination-promoting activity.
在碱提取-酸沉淀这一常见步骤的基础上,人们开发了褐煤处理技术,以提高腐植酸(HA)的提取效率和产量。本研究探讨了将粘土拟杆菌(P.m)LT1906 和真菌拟青霉(P.p)LL2205 菌株结合起来,对采集自内蒙古东部的褐煤进行前处理和后处理,以提高腐殖酸和富里酸(FA)的产量以及促进植物生长的活性。分别对褐煤进行了先P.m后HNO3处理、先HNO3后P.p处理以及P.m、HNO3和P.p组合处理,然后采用碱提取-酸沉淀技术进行处理。结果表明,P.m 预处理使 HA 产量提高了 12.47%,而 P.p 后处理则使 FA 产量显著提高了 13.37%。细菌和真菌联合处理显著提高了 HA 和 FA 的总产量,提高了 19.12%。结构分析表明,P.m 预处理导致褐煤表面变形,褐煤完全氧化并发生浸出反应,从而提高了 HA 的产量,但对 HA 的元素组成和化学结构没有影响。P.p 后处理通过褐煤的氧化解聚作用引入了含氧官能团并减少了芳香成分,从而提高了 FA 的产量和 HA 的种子发芽促进活性。
期刊介绍:
Process Biochemistry is an application-orientated research journal devoted to reporting advances with originality and novelty, in the science and technology of the processes involving bioactive molecules and living organisms. These processes concern the production of useful metabolites or materials, or the removal of toxic compounds using tools and methods of current biology and engineering. Its main areas of interest include novel bioprocesses and enabling technologies (such as nanobiotechnology, tissue engineering, directed evolution, metabolic engineering, systems biology, and synthetic biology) applicable in food (nutraceutical), healthcare (medical, pharmaceutical, cosmetic), energy (biofuels), environmental, and biorefinery industries and their underlying biological and engineering principles.