Advancements in Multiple-Step On-Line Preconcentration Techniques for Enhanced Sensitivity in Capillary Electrophoresis

IF 2.8 3区工程技术 Q2 CHEMISTRY, ANALYTICAL

Journal of separation science Pub Date : 2024-09-20 DOI:10.1002/jssc.202400519

Aemi Syazwani Abdul Keyon, NyukTing Ng, Michael Charles Breadmore

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Abstract

Multiple-step on-line preconcentration, a combination of at least two stacking techniques has been developed to increase the sensitivity in capillary electrophoresis (CE) for analytes in various samples. It is usually conducted sequentially, or in some cases, synergistically, where different stacking modes occur simultaneously. Multiple-step techniques allow simultaneous preconcentration and separation of various kinds of analytes in different complex samples in a single CE run. This review aims to provide recent advances in multiple-step on-line preconcentration techniques in CE. We critically review technical papers published for the last 7 years up until July 2024, subsequently organized according to the combination of the main stacking techniques, that is, field amplification, large volume sample stacking, transient isotachophoresis, micelle to solvent or micelle to cyclodextrin stacking, and others. The procedures, fundamental mechanism, analytical figures of merits achieved, and their feasibility for complicated sample matrices are reviewed.

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提高毛细管电泳灵敏度的多步在线预浓缩技术的进展

为了提高毛细管电泳（CE）对各种样品中分析物的灵敏度，人们开发了多步在线预浓缩技术，该技术结合了至少两种叠加技术。它通常是按顺序进行的，或者在某些情况下是协同进行的，即不同的叠加模式同时进行。多步骤技术允许在一次 CE 运行中同时预浓缩和分离不同复杂样品中的各种分析物。本综述旨在介绍 CE 中多步在线预浓缩技术的最新进展。我们对截至 2024 年 7 月的近 7 年发表的技术论文进行了严格审查，随后根据主要堆积技术（即场放大、大体积样品堆积、瞬时等渗电泳、胶束到溶剂或胶束到环糊精堆积等）的组合进行了整理。本文综述了这些技术的操作步骤、基本机理、取得的分析成果以及它们在复杂样品基质中的可行性。

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来源期刊

Journal of separation science 化学-分析化学

CiteScore

6.30

自引率

16.10%

发文量

408

审稿时长

1.8 months

期刊介绍： The Journal of Separation Science (JSS) is the most comprehensive source in separation science, since it covers all areas of chromatographic and electrophoretic separation methods in theory and practice, both in the analytical and in the preparative mode, solid phase extraction, sample preparation, and related techniques. Manuscripts on methodological or instrumental developments, including detection aspects, in particular mass spectrometry, as well as on innovative applications will also be published. Manuscripts on hyphenation, automation, and miniaturization are particularly welcome. Pre- and post-separation facets of a total analysis may be covered as well as the underlying logic of the development or application of a method.