Functional Muffins Exert Bifidogenic Effects along with Highly Product-Specific Effects on the Human Gut Microbiota Ex Vivo

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Metabolites Pub Date : 2024-09-14 DOI:10.3390/metabo14090497

Stef Deyaert, Jonas Poppe, Lam Dai Vu, Aurélien Baudot, Sarah Bubeck, Thomas Bayne, Kiran Krishnan, Morgan Giusto, Samuel Moltz, Pieter Van den Abbeele

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引用次数: 0

Abstract

GoodBiome™ Foods are functional foods containing a probiotic (Bacillus subtilis HU58™) and prebiotics (mainly inulin). Their effects on the human gut microbiota were assessed using ex vivo SIFR® technology, which has been validated to provide clinically predictive insights. GoodBiome™ Foods (BBM/LCM/OSM) were subjected to oral, gastric, and small intestinal digestion/absorption, after which their impact on the gut microbiome of four adults was assessed (n = 3). All GoodBiome™ Foods boosted health-related SCFA acetate (+13.1/14.1/13.8 mM for BBM/LCM/OSM), propionate (particularly OSM; +7.4/7.5/8.9 mM for BBM/LCM/OSM) and butyrate (particularly BBM; +2.6/2.1/1.4 mM for BBM/LCM/OSM). This is related to the increase in Bifidobacterium species (B. catenulatum, B. adolescentis, B. pseudocatenulatum), Coprococcus catus and Bacteroidetes members (Bacteroides caccae, Phocaeicola dorei, P. massiliensis), likely mediated via inulin. Further, the potent propionogenic potential of OSM related to increased Bacteroidetes members known to ferment oats (s key ingredient of OSM), while the butyrogenic potential of BBM related to a specific increase in Anaerobutyricum hallii, a butyrate producer specialized in the fermentation of erythritol (key ingredient of BBM). In addition, OSM/BBM suppressed the pathogen Clostridioides difficile, potentially due to inclusion of HU58™ in GoodBiome™ Foods. Finally, all products enhanced a spectrum of metabolites well beyond SCFA, including vitamins (B3/B6), essential amino acids, and health-related metabolites such as indole-3-propionic acid. Overall, the addition of specific ingredients to complex foods was shown to specifically modulate the gut microbiome, potentially contributing to health benefits. Noticeably, our findings contradict a recent in vitro study, underscoring the critical role of employing a physiologically relevant digestion/absorption procedure for a more accurate evaluation of the microbiome-modulating potential of complex foods.

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功能性松饼在体内对人体肠道微生物群产生双歧因子效应和高度产品特异性效应

GoodBiome™ 食品是含有益生菌（枯草芽孢杆菌 HU58™）和益生元（主要是菊粉）的功能性食品。它们对人体肠道微生物群的影响采用体内外 SIFR® 技术进行评估，该技术已通过验证，可提供临床预测性见解。对 GoodBiome™ 食品（BBM/LCM/OSM）进行口腔、胃和小肠消化/吸收，然后评估它们对四名成年人（n = 3）肠道微生物群的影响。所有 GoodBiome™ 食品都提高了与健康相关的 SCFA 醋酸酯（BBM/LCM/OSM 为 +13.1/14.1/13.8 mM）、丙酸酯（尤其是 OSM；BBM/LCM/OSM 为 +7.4/7.5/8.9 mM）和丁酸酯（尤其是 BBM；BBM/LCM/OSM 为 +2.6/2.1/1.4 mM）。这与双歧杆菌（B. catenulatum、B. adolescentis、B. pseudocatenulatum）、猫双歧杆菌（Coprococcus catus）和类杆菌（Bacteroides caccae、Phocaeicola dorei、P. massiliensis）的增加有关，可能是通过菊粉介导的。此外，OSM 的强力产丙酸潜能与已知可发酵燕麦（OSM 的主要成分）的类杆菌属成员增加有关，而 BBM 的产丁酸潜能则与 Anaerobutyricum hallii（一种专门从事赤藓糖醇（BBM 的主要成分）发酵的丁酸生产者）的特定增加有关。此外，OSM/BBM 还能抑制艰难梭菌，这可能是由于 GoodBiome™ 食品中含有 HU58™。最后，所有产品都提高了SCFA以外的一系列代谢物，包括维生素（B3/B6）、必需氨基酸和与健康相关的代谢物，如吲哚-3-丙酸。总之，在复合食品中添加特定成分可对肠道微生物组产生特定的调节作用，从而对健康产生潜在的益处。值得注意的是，我们的研究结果与最近的一项体外研究相矛盾，强调了采用与生理相关的消化/吸收程序对于更准确地评估复合食品的微生物组调节潜力的关键作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Metabolites Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

5.70

自引率

7.30%

发文量

1070

审稿时长

17.17 days

期刊介绍： Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.