Ning Yan, Junfeng Cao, Jie Wang, Xiaoxia Zou, Xiaona Yu, Xiaojun Zhang, Tong Si
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引用次数: 0
Abstract
Graphene oxide (GO), beyond its specialized industrial applications, is rapidly gaining prominence as a nanomaterial for modern agriculture. However, its specific effects on seed priming for salinity tolerance and yield formation in crops remain elusive. Under both pot-grown and field-grown conditions, this study combined physiological indices with transcriptomics and metabolomics to investigate how GO affects seed germination, seedling salinity tolerance, and peanut pod yield. Peanut seeds were firstly treated with 400 mg L⁻¹ GO (termed GO priming). At seed germination stage, GO-primed seeds exhibited higher germination rate and percentage of seeds with radicals breaking through the testa. Meanwhile, omics analyses revealed significant enrichment in pathways associated with carbon and nitrogen metabolisms in GO-primed seeds. At seedling stage, GO priming contributed to strengthening plant growth, enhancing photosynthesis, maintaining the integrity of plasma membrane, and promoting the nutrient accumulation in peanut seedlings under 200 mM NaCl stress. Moreover, GO priming increased the activities of antioxidant enzymes, along with reduced the accumulation of reactive oxygen species (ROS) in response to salinity stress. Furthermore, the differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs) of peanut seedlings under GO priming were mainly related to photosynthesis, phytohormones, antioxidant system, and carbon and nitrogen metabolisms in response to soil salinity. At maturity, GO priming showed an average increase in peanut pod yield by 12.91% compared with non-primed control. Collectively, our findings demonstrated that GO plays distinguish roles in enhancing seed germination, mitigating salinity stress, and boosting pod yield in peanut plants via modulating multiple physiological processes.
氧化石墨烯(GO)除了其专业的工业应用外,还作为一种现代农业纳米材料迅速受到重视。然而,氧化石墨烯对农作物种子耐盐性和产量形成的具体影响仍然难以捉摸。在盆栽和大田种植条件下,本研究将生理指标与转录组学和代谢组学相结合,研究 GO 如何影响种子萌发、幼苗耐盐性和花生荚果产量。首先用 400 mg L-¹ GO 处理花生种子(称为 GO 引物)。在种子萌发阶段,GO-primed 种子表现出更高的萌发率和自由基突破种皮的种子百分比。同时,全局分析表明,GO-primed 种子中与碳和氮代谢相关的通路显著富集。在幼苗期,GO引物有助于在200 mM NaCl胁迫下加强花生幼苗的生长、提高光合作用、维持质膜完整性和促进营养积累。此外,GO引物提高了抗氧化酶的活性,并减少了盐胁迫下活性氧(ROS)的积累。此外,GO引物作用下花生幼苗的差异表达基因(DEGs)和差异积累代谢物(DAMs)主要与光合作用、植物激素、抗氧化系统、碳氮代谢等有关。在花生成熟期,GO 引种比非引种对照的花生荚果产量平均增加了 12.91%。总之,我们的研究结果表明,GO 通过调节多种生理过程,在提高种子萌发、减轻盐分胁迫和提高花生荚果产量方面发挥着不同的作用。
期刊介绍:
Journal of Nanobiotechnology is an open access peer-reviewed journal communicating scientific and technological advances in the fields of medicine and biology, with an emphasis in their interface with nanoscale sciences. The journal provides biomedical scientists and the international biotechnology business community with the latest developments in the growing field of Nanobiotechnology.