Synergistic xylan decomposition by a reducing-end xylose-releasing exo-oligoxylanase with other xylanolytic enzymes derived from Paenibacillus xylaniclasticus strain TW1.

IF 1.4 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-09-18 DOI:10.1093/bbb/zbae130

Koki Taniguchi,Shuichi Karita,Midori Umekawa

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引用次数: 0

Abstract

Paenibacillus xylaniclasticus strain TW1 is a promising tool for decomposing xylan-containing lignocellulosic biomass, since this strain possesses various genes encoding cellulolytic/hemicellulolytic enzymes. In this study, PxRex8A from the TW1 strain was found to be a reducing-end xylose-releasing exo-oligoxylanase of glycoside hydrolase family 8, which cleaves xylose from xylooligosacchrides of corn core xylan. In synergistic assay, the efficient decomposition of oat spelt xylan (OSX) and beech wood xylan was exemplified in the combination of endo β-1,4-xylanase (PxXyn11A) and PxRex8A from the TW1 strain in a molar ratio of 4:1. Furthermore, it was found that the addition of β-d-xylosidase/α-l-arabinofuranosidase (PxXyl43A) from this strain with PxXyn11A and PxRex8A achieved twice the amount of reducing sugars (1.1 mg/mL) against OSX after 24 hours compared to PxXyn11A alone (0.5 mg/mL). These results present that synergy effect of PxRex8A and PxXyl43A with PxXyn11A promotes xylan degradation into xylose.

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一种还原端木糖释放外低聚木糖酶与其他木糖分解酶协同分解木糖，这些酶来自木糖胶冻芽孢杆菌（Paenibacillus xylaniclasticus）菌株 TW1。

木聚糖酵母菌（Paenibacillus xylaniclasticus）菌株 TW1 是分解含木聚糖木质纤维素生物质的有效工具，因为该菌株拥有多种编码纤维素分解酶/半纤维素分解酶的基因。本研究发现，TW1 菌株的 PxRex8A 是糖苷水解酶家族 8 的还原端木糖释放外低聚氧乙烯醚酶，可将木糖从玉米核心木聚糖的外低聚氧乙烯醚中裂解出来。在协同作用试验中，将来自 TW1 菌株的内β-1,4-木聚糖酶（PxXyn11A）和 PxRex8A 以 4:1 的摩尔比结合使用，可有效分解燕麦斯佩耳特木聚糖（OSX）和榉木木聚糖。此外，研究还发现，与单独使用 PxXyn11A（0.5 毫克/毫升）相比，在 24 小时后，将该菌株的 β-d- 木糖苷酶/α-阿拉伯呋喃糖苷酶（PxXyl43A）与 PxXyn11A 和 PxRex8A 混合使用，可使还原糖（1.1 毫克/毫升）对 OSX 的还原量增加一倍。这些结果表明，PxRex8A 和 PxXyl43A 与 PxXyn11A 的协同作用可促进木聚糖降解为木糖。

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来源期刊

Bioscience, Biotechnology, and Biochemistry 生物-生化与分子生物学

CiteScore

3.50

自引率

0.00%

发文量

183

审稿时长

1 months

期刊介绍： Bioscience, Biotechnology, and Biochemistry publishes high-quality papers providing chemical and biological analyses of vital phenomena exhibited by animals, plants, and microorganisms, the chemical structures and functions of their products, and related matters. The Journal plays a major role in communicating to a global audience outstanding basic and applied research in all fields subsumed by the Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA).