Morphological, Physiological, and Biochemical Characteristics of the Sarcinoid Microalga Chlorosarcinopsis eremi (Chlorophyceae, Chlorophyta)

IF 1.1 4区 生物学 Q3 PLANT SCIENCES
N. V. Dantsyuk, I. N. Chubchikova, A. D. Temraleeva, G. S. Minyuk, I. V. Drobetskaya
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Abstract

The strain VKM Al-132 of green sarcinoid microalgae isolated from chestnut soil of the dry steppe zone (Volgograd oblast of Russia) was investigated. With the help of light- and scanning electron microscopy, together with the molecular phylogenetic analysis of the 18S rRNA gene and the ITS2 spacer, the strain was identified as Chlorosarcinopsis eremi. The characteristics of its growth and secondary carotenogenesis were examined under conditions of two-stage batch culture. After 21-day cultivation, the average productivity was 0.12 g/(L day) (dry biomass) and 0.2 mg/(L day) (total carotenoids). At the end of the stage of secondary carotenogenesis, when the percentage of total carotenoids was approximately 0.25% of dry biomass, canthaxanthin and diesters of astaxanthin fractions dominated; the sum of astaxanthin esters attained 36% of total carotenoids. It has been shown that the VKM Al-132 strain of C. eremi may serve as a potentially perspective object to search for optimal conditions for intensification of carotenoid biosynthesis.

Abstract Image

拟石蒜科微小藻类 Chlorosarcinopsis eremi(叶绿藻科,叶绿藻属)的形态、生理和生化特征
摘要 研究了从干草原区(俄罗斯伏尔加格勒州)栗树土壤中分离出的绿色石炭酸微藻菌株 VKM Al-132。借助光镜和扫描电子显微镜以及 18S rRNA 基因和 ITS2 spacer 的分子系统学分析,确定该菌株为 Chlorosarcinopsis eremi。在两阶段分批进行培养的条件下,研究了其生长和次生胡萝卜素生成的特点。经过 21 天的培养,平均产量为 0.12 克/(升/天)(干生物量)和 0.2 毫克/(升/天)(类胡萝卜素总量)。在次生类胡萝卜素生成阶段结束时,类胡萝卜素总量约占干生物量的 0.25%,其中主要是角黄素和虾青素二酯;虾青素酯的总和达到类胡萝卜素总量的 36%。研究结果表明,VKM Al-132 勃氏海藻菌株可作为寻找类胡萝卜素生物合成最佳强化条件的潜在研究对象。
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来源期刊
CiteScore
4.00
自引率
14.30%
发文量
107
审稿时长
6 months
期刊介绍: Russian Journal of Plant Physiology is a leading journal in phytophysiology. It embraces the full spectrum of plant physiology and brings together the related aspects of biophysics, biochemistry, cytology, anatomy, genetics, etc. The journal publishes experimental and theoretical articles, reviews, short communications, and descriptions of new methods. Some issues cover special problems of plant physiology, thus presenting collections of articles and providing information in rapidly growing fields. The editorial board is highly interested in publishing research from all countries and accepts manuscripts in English.
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