Transcriptional regulation of phospholipid transport in cotton fiber elongation by GhMYB30D04–GhHD1 interaction complex

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Qingwei Song, Chuanhui Du, Yiyang Xu, Jin Wang, Min Lin, Kaijing Zuo
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引用次数: 0

Abstract

Cotton fiber length is basically determined by well‐coordinated gene expression and phosphatidylinositol phosphates (PIPs) accumulation during fiber elongation but the regulatory mechanism governing PIPs transport remains unknown. Here, we report a MYB transcription factor GhMYB30D04 in Gossypium hirsutum that promotes fiber elongation through modulating the expression of PIP transporter gene GhLTPG1. Knockout of GhMYB30D04 gene in cotton (KO) results in a reduction of GhLTPG1 transcripts with lower accumulation of PIPs, leading to shorter fibers and lower fiber yield. Conversely, GhMYB30D04 overexpression (GhMYB30D04‐OE) causes richer PIPs and longer cotton fibers, mimicking the effects of exogenously applying PIPs on the ovules of GhMYB30D04‐KO and wild type. Furthermore, GhMYB30D04 interacts with GhHD1, the crucial transcription factor of fiber initiation, to form an activation complex stabilized by PIPs, both of which upregulate GhLTPG1 expression. Comparative omics‐analysis revealed that higher and extended expressions of LTPG1 in fiber elongation mainly correlate with the variations of the GhMYB30D04 gene between two cotton allotetraploids, contributing to longer fiber in G. babardense. Our work clarifies a mechanism by which GhHD1–GhMYB30D04 form a regulatory module of fiber elongation to tightly control PIP accumulation. Our work still has an implication that GhMYB30D04–GhHD1 associates with development transition from fiber initiation to elongation.
GhMYB30D04-GhHD1相互作用复合物对棉纤维伸长过程中磷脂运输的转录调控
棉花纤维长度基本上是由纤维伸长过程中协调良好的基因表达和磷脂酰肌醇磷酸酯(PIPs)积累决定的,但 PIPs 转运的调控机制仍不清楚。在这里,我们报告了一种 MYB 转录因子 GhMYB30D04,它通过调节 PIP 转运基因 GhLTPG1 的表达来促进纤维伸长。在棉花中敲除 GhMYB30D04 基因(KO)会导致 GhLTPG1 转录本减少,PIPs 积累降低,从而导致纤维变短,纤维产量降低。相反,GhMYB30D04 过表达(GhMYB30D04-OE)会导致更丰富的 PIPs 和更长的棉纤维,模拟了外源施加 PIPs 对 GhMYB30D04-KO 和野生型胚珠的影响。此外,GhMYB30D04 与纤维起始的关键转录因子 GhHD1 相互作用,形成由 PIPs 稳定的活化复合物,两者都能上调 GhLTPG1 的表达。omics 比较分析表明,LTPG1 在纤维伸长过程中的高表达和延长表达主要与两个棉花异源四倍体之间 GhMYB30D04 基因的变异有关,这有助于延长 G. babardense 的纤维。我们的工作阐明了 GhHD1-GhMYB30D04 形成纤维伸长调控模块以严格控制 PIP 积累的机制。我们的研究还表明,GhMYB30D04-GhHD1 与纤维起始到伸长的发育转变有关。
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来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
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