Qingwei Song, Chuanhui Du, Yiyang Xu, Jin Wang, Min Lin, Kaijing Zuo
{"title":"Transcriptional regulation of phospholipid transport in cotton fiber elongation by GhMYB30D04–GhHD1 interaction complex","authors":"Qingwei Song, Chuanhui Du, Yiyang Xu, Jin Wang, Min Lin, Kaijing Zuo","doi":"10.1111/jipb.13776","DOIUrl":null,"url":null,"abstract":"Cotton fiber length is basically determined by well‐coordinated gene expression and phosphatidylinositol phosphates (PIPs) accumulation during fiber elongation but the regulatory mechanism governing PIPs transport remains unknown. Here, we report a MYB transcription factor GhMYB30D04 in <jats:italic>Gossypium hirsutum</jats:italic> that promotes fiber elongation through modulating the expression of PIP transporter gene <jats:italic>GhLTPG1</jats:italic>. Knockout of <jats:italic>GhMYB30D04</jats:italic> gene in cotton (KO) results in a reduction of <jats:italic>GhLTPG1</jats:italic> transcripts with lower accumulation of PIPs, leading to shorter fibers and lower fiber yield. Conversely, <jats:italic>GhMYB30D04</jats:italic> overexpression (<jats:italic>GhMYB30D04‐OE</jats:italic>) causes richer PIPs and longer cotton fibers, mimicking the effects of exogenously applying PIPs on the ovules of <jats:italic>GhMYB30D04‐KO</jats:italic> and wild type. Furthermore, GhMYB30D04 interacts with GhHD1, the crucial transcription factor of fiber initiation, to form an activation complex stabilized by PIPs, both of which upregulate <jats:italic>GhLTPG1</jats:italic> expression. Comparative omics‐analysis revealed that higher and extended expressions of <jats:italic>LTPG1</jats:italic> in fiber elongation mainly correlate with the variations of the <jats:italic>GhMYB30D04</jats:italic> gene between two cotton allotetraploids, contributing to longer fiber in <jats:italic>G. babardense</jats:italic>. Our work clarifies a mechanism by which GhHD1–GhMYB30D04 form a regulatory module of fiber elongation to tightly control PIP accumulation. Our work still has an implication that GhMYB30D04–GhHD1 associates with development transition from fiber initiation to elongation.","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"9 1","pages":""},"PeriodicalIF":9.3000,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Integrative Plant Biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1111/jipb.13776","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Cotton fiber length is basically determined by well‐coordinated gene expression and phosphatidylinositol phosphates (PIPs) accumulation during fiber elongation but the regulatory mechanism governing PIPs transport remains unknown. Here, we report a MYB transcription factor GhMYB30D04 in Gossypium hirsutum that promotes fiber elongation through modulating the expression of PIP transporter gene GhLTPG1. Knockout of GhMYB30D04 gene in cotton (KO) results in a reduction of GhLTPG1 transcripts with lower accumulation of PIPs, leading to shorter fibers and lower fiber yield. Conversely, GhMYB30D04 overexpression (GhMYB30D04‐OE) causes richer PIPs and longer cotton fibers, mimicking the effects of exogenously applying PIPs on the ovules of GhMYB30D04‐KO and wild type. Furthermore, GhMYB30D04 interacts with GhHD1, the crucial transcription factor of fiber initiation, to form an activation complex stabilized by PIPs, both of which upregulate GhLTPG1 expression. Comparative omics‐analysis revealed that higher and extended expressions of LTPG1 in fiber elongation mainly correlate with the variations of the GhMYB30D04 gene between two cotton allotetraploids, contributing to longer fiber in G. babardense. Our work clarifies a mechanism by which GhHD1–GhMYB30D04 form a regulatory module of fiber elongation to tightly control PIP accumulation. Our work still has an implication that GhMYB30D04–GhHD1 associates with development transition from fiber initiation to elongation.
期刊介绍:
Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.