The effects of humic substances application on the phytohormone profile in Lactuca sativa L.

IF 2.2 3区农林科学 Q2 AGRICULTURE, MULTIDISCIPLINARY

Annals of Applied Biology Pub Date : 2024-09-14 DOI:10.1111/aab.12944

Santiago Atero‐Calvo, Francesco Magro, Giacomo Masetti, Eloy Navarro‐León, Alfonso Albacete, Juan Manuel Ruiz

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引用次数: 0

Abstract

Humic substances (HS) are commonly employed as plant biostimulants to enhance crop yields. However, the HS mechanisms of action, as well as the differences between radicular and foliar modes of application, remain unclear. Here, we explored the changes in phytohormonal balance as possible mechanism of HS to enhance lettuce (Lactuca sativa L.) growth, and the difference between both modes of application. For this purpose, BLACKJAK®, a HS‐based product was applied as radicular (R) and foliar (F) at the concentrations (mL/L): 0.20 (R1), 0.40 (R2), 0.60 (R3), 0.80 (R4), 5.00 (F1), 7.50 (F2), 10.00 (F3), and 12.50 (F4). The experiment was performed in pots filled with vermiculite:perlite (3:1) and HS were applied three times with a periodicity of 10 days. Shoot and root growth parameters were measured. In addition, the phythormones indole‐3‐acetic acid (IAA), gibberellins (GAs), trans‐zeatine (tZ), isopentenyl adenine (iP), 1‐aminocyclopropane‐1‐carboxylic acid (ACC), abscisic acid (ABA), jasmonic acid (JA), and salicylic acid (SA), were analysed by U‐HPLC‐MS. BLACKJAK® application resulted in higher shoot growth at doses R1, R2, R3, F2, and F3, whereas root biomass was increased at R2, R3, F2, F3, and F4, showing radicular better plant growth than foliar applications. Furthermore, HS changed phytohormonal balance in shoots and roots. However, it was with radicular applications, especially at R2, where phytohormonal profile was best associated with plant growth due to the increases observed in IAA, GAs, JA, SA, tZ, and decreased ABA. However, further research is needed to clarify the involvement of hormones in the growth‐promoting action of HS.

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施用腐殖质对 Lactuca sativa L 植物激素谱的影响

腐殖质（HS）通常被用作植物生物刺激剂，以提高作物产量。然而，HS 的作用机制以及径向施用和叶面施用模式之间的差异仍不清楚。在此，我们探讨了植物激素平衡的变化作为 HS 促进莴苣（Lactuca sativa L.）生长的可能机制，以及两种施用模式之间的差异。为此，使用了基于 HS 的产品 BLACKJAK®，使用浓度（毫升/升）分别为 0.20（R1）、0.40（R2）、0.60（R3）、0.80（R4）、5.00（F1）、7.50（F2）、10.00（F3）和 12.50（F4）。实验在装有蛭石和珍珠岩（3:1）的花盆中进行，HS 施用三次，周期为 10 天。测量了芽和根的生长参数。此外，还通过 U-HPLC-MS 分析了植物激素吲哚-3-乙酸（IAA）、赤霉素（GAs）、反式玉米素（tZ）、异戊烯基腺嘌呤（iP）、1-氨基环丙烷-1-羧酸（ACC）、脱落酸（ABA）、茉莉酸（JA）和水杨酸（SA）。施用 BLACKJAK® 后，R1、R2、R3、F2 和 F3 剂量的嫩枝生长量增加，而 R2、R3、F2、F3 和 F4 剂量的根生物量增加，这表明径向施用比叶面施用更有利于植物生长。此外，HS 改变了芽和根的植物激素平衡。然而，在径向施用时，尤其是在 R2，植物激素谱与植物生长的关系最好，因为观察到 IAA、GA、JA、SA、tZ 增加，而 ABA 减少。不过，要弄清激素在 HS 生长促进作用中的参与情况，还需要进一步的研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Annals of Applied Biology 生物-农业综合

CiteScore

5.50

自引率

0.00%

发文量

审稿时长

18-36 weeks

期刊介绍： Annals of Applied Biology is an international journal sponsored by the Association of Applied Biologists. The journal publishes original research papers on all aspects of applied research on crop production, crop protection and the cropping ecosystem. The journal is published both online and in six printed issues per year. Annals papers must contribute substantially to the advancement of knowledge and may, among others, encompass the scientific disciplines of: Agronomy Agrometeorology Agrienvironmental sciences Applied genomics Applied metabolomics Applied proteomics Biodiversity Biological control Climate change Crop ecology Entomology Genetic manipulation Molecular biology Mycology Nematology Pests Plant pathology Plant breeding & genetics Plant physiology Post harvest biology Soil science Statistics Virology Weed biology Annals also welcomes reviews of interest in these subject areas. Reviews should be critical surveys of the field and offer new insights. All papers are subject to peer review. Papers must usually contribute substantially to the advancement of knowledge in applied biology but short papers discussing techniques or substantiated results, and reviews of current knowledge of interest to applied biologists will be considered for publication. Papers or reviews must not be offered to any other journal for prior or simultaneous publication and normally average seven printed pages.