Neutrophil phenotype, effector functions and microbicidal activity in SARS-CoV-2-associated ARDS patients.

IF 3.6 3区医学 Q3 CELL BIOLOGY

Journal of Leukocyte Biology Pub Date : 2024-09-12 DOI:10.1093/jleuko/qiae195

Quentin Quelven,Murielle Grégoire,Valentin Coirier,Arnaud Gacouin,Simon Le Gallou,Vincent Cattoir,Michel Cogné,Hélène Guegan,Jean-Pierre Gangneux,Mikael Roussel,Karin Tarte,Jean-Marc Tadié,Mathieu Lesouhaitier

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引用次数: 0

Abstract

Critically ill patients admitted to the intensive care unit (ICU) for SARS-CoV-2-induced acute respiratory distress syndrome (ARDS) are at increased risk of bacterial and fungal secondary pulmonary infections due to acquired immune dysfunction. Given that the activity of neutrophils has not been described in these patients, we aimed to investigate the function of neutrophils at ICU admission and on Day 7 (D7) post admission. Neutrophil maturation and several functional indicators were investigated. We detected a significant decrease in reactive oxygen species production at D7, but we did not observe any other significant alterations in neutrophil function. Furthermore, bronchoalveolar lavage obtained from patients displayed no inhibitory effect on the function of neutrophils from healthy donors. These findings indicate that patients admitted to the ICU for SARS-CoV-2-induced ARDS do not acquire neutrophil dysfunction within the first week of their stay, which suggests that nosocomial infections among these patients are not due to acquired neutrophil dysfunctions.

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SARS-CoV-2 相关 ARDS 患者的中性粒细胞表型、效应功能和杀微生物活性。

因 SARS-CoV-2 引发的急性呼吸窘迫综合征（ARDS）而住进重症监护室（ICU）的重症患者由于后天免疫功能障碍而增加了继发细菌和真菌肺部感染的风险。鉴于中性粒细胞的活性尚未在这些患者中得到描述，我们的目的是研究中性粒细胞在进入 ICU 和入院后第 7 天（D7）的功能。我们对中性粒细胞的成熟度和几项功能指标进行了调查。我们发现在第 7 天活性氧的产生明显减少，但我们没有观察到中性粒细胞功能的任何其他明显变化。此外，患者的支气管肺泡灌洗液对健康供体的中性粒细胞功能没有抑制作用。这些研究结果表明，因 SARS-CoV-2 引起的 ARDS 而入住重症监护室的患者在住院的第一周内不会出现中性粒细胞功能障碍，这表明这些患者的院内感染并非由于后天中性粒细胞功能障碍所致。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Leukocyte Biology 医学-免疫学

CiteScore

11.50

自引率

0.00%

发文量

358

审稿时长

2 months

期刊介绍： JLB is a peer-reviewed, academic journal published by the Society for Leukocyte Biology for its members and the community of immunobiologists. The journal publishes papers devoted to the exploration of the cellular and molecular biology of granulocytes, mononuclear phagocytes, lymphocytes, NK cells, and other cells involved in host physiology and defense/resistance against disease. Since all cells in the body can directly or indirectly contribute to the maintenance of the integrity of the organism and restoration of homeostasis through repair, JLB also considers articles involving epithelial, endothelial, fibroblastic, neural, and other somatic cell types participating in host defense. Studies covering pathophysiology, cell development, differentiation and trafficking; fundamental, translational and clinical immunology, inflammation, extracellular mediators and effector molecules; receptors, signal transduction and genes are considered relevant. Research articles and reviews that provide a novel understanding in any of these fields are given priority as well as technical advances related to leukocyte research methods.