Fabrication of In Situ-Cross-Linked N-Succinyl Chitosan/Oxidized Alginate Hydrogel-Loaded Ascorbic Acid and Biphasic Calcium Phosphate for Bone Tissue Engineering

IF 3.2 4区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biopolymers Pub Date : 2024-09-20 DOI:10.1002/bip.23628

Thanh-Thuong Le Duong, Binh Thanh Vu, Hanh Thi-Kieu Ta, Quan Minh Vo, Thanh Dinh Le, Thi-Hiep Nguyen

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Abstract

Bone tissue engineering is a promising technology being studied globally to become an effective and sustainable method to treat the problems of damaged or diseased bones. In this work, we developed an in situ cross-linking hydrogel system that combined N-succinyl chitosan (NSC) and oxidized alginate (OA) at varying mixing ratios through Schiff base cross-linking. The hydrogel system also contains biphasic calcium phosphate (BCP) and ascorbic acid (AA), which could enhance biological characteristics and accelerate bone repair. The hydrogels' properties were examined through physicochemical tests such as scanning electron microscopy (SEM), energy-dispersive x-ray spectroscopy (EDS), Fourier transform infrared spectroscopy (FT-IR), x-ray diffraction (XRD), pore size and porosity measurement, swelling ratio, degradation rate, AA release study, as well as cytocompatibility, including live/dead and cytotoxicity assays. The results revealed that the supplementation of AA and BCP components can affect the physico-mechanical properties of the hydrogel system. However, they exhibited noncytotoxic properties. Overall, the results demonstrated that the hydrogel composed of 3% (w/v) NSC and 3% (w/v) OA (NSC: OA volume ratio is 8:2) loaded with 40% (w/w) BCP and 0.3 mg/mL AA has the potential for bone regeneration.

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用于骨组织工程的原位交联 N-琥珀酰壳聚糖/氧化海藻酸水凝胶负载抗坏血酸和双相磷酸钙的制备方法

骨组织工程是一项前景广阔的技术，正在全球范围内进行研究，它将成为治疗受损或病变骨骼问题的一种有效且可持续的方法。在这项工作中，我们开发了一种原位交联水凝胶系统，通过席夫碱交联将 N-琥珀酰壳聚糖（NSC）和氧化海藻酸盐（OA）以不同的混合比例结合在一起。该水凝胶系统还含有双相磷酸钙（BCP）和抗坏血酸（AA），可增强生物特性并加速骨修复。研究人员通过扫描电子显微镜（SEM）、能量色散 X 射线光谱（EDS）、傅立叶变换红外光谱（FT-IR）、X 射线衍射（XRD）、孔径和孔隙率测量、膨胀率、降解率、AA 释放研究以及细胞相容性（包括活/死和细胞毒性试验）等理化测试检验了水凝胶的特性。结果表明，添加 AA 和 BCP 成分会影响水凝胶体系的物理机械性能。不过，它们具有无细胞毒性的特性。总之，研究结果表明，由 3% (w/v) NSC 和 3% (w/v) OA（NSC：OA 体积比为 8:2）与 40% (w/w) BCP 和 0.3 mg/mL AA 组成的水凝胶具有骨再生的潜力。

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来源期刊

Biopolymers 生物-生化与分子生物学

CiteScore

5.30

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： Founded in 1963, Biopolymers publishes strictly peer-reviewed papers examining naturally occurring and synthetic biological macromolecules. By including experimental and theoretical studies on the fundamental behaviour as well as applications of biopolymers, the journal serves the interdisciplinary biochemical, biophysical, biomaterials and biomedical research communities.