Shuji Gao, Shuo Yuan, Yingying Quan, Wenjie Jin, Yamin Shen, Baobao Liu, Yuxin Wang, Yang Wang
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引用次数: 0
Abstract
Biofilm is the primary cause of persistent infections caused by Streptococcus suis (S. suis). Metabolism and AI-2 quorum sensing are intricately linked to S. suis biofilm formation. Although the role of the AI-2 quorum sensing luxS gene in S. suis biofilm has been reported, its specific regulatory mechanism remains unclear. This study explored the differences in biofilm formation and monosaccharide metabolism among the wild type (WT), luxS mutant (ΔluxS) and complement strain (CΔluxS), and Galleria mellonella larvae were used to access the effect of luxS gene deletion on the virulence of S. suis in different monosaccharide medias. The results indicated that deletion of the luxS gene further compromised the monosaccharide metabolism of S. suis, impacting its growth in media with fructose, galactose, rhamnose, and mannose as the sole carbon sources. However, no significant impact was observed in media with glucose and N-acetylglucosamine. This deletion also weakened EPS synthesis, thereby diminishing the biofilm formation capacity of S. suis. Additionally, the downregulation of adhesion gene expression due to luxS gene deletion was found to be independent of the monosaccharide medias of S. suis.
生物膜是猪链球菌(S. suis)引起持续感染的主要原因。新陈代谢和AI-2法定人数感应与猪链球菌生物膜的形成密切相关。虽然有报道称AI-2法定人数感应luxS基因在猪链球菌生物膜中发挥作用,但其具体的调控机制仍不清楚。本研究探讨了野生型(WT)、luxS突变体(ΔluxS)和互补株(CΔluxS)之间在生物膜形成和单糖代谢方面的差异,并利用Galleria mellonella幼虫研究了luxS基因缺失对鼠伤寒杆菌在不同单糖介质中毒力的影响。结果表明,缺失luxS基因会进一步损害猪链球菌的单糖代谢,影响其在以果糖、半乳糖、鼠李糖和甘露糖为唯一碳源的培养基中的生长。然而,在含有葡萄糖和 N-乙酰葡糖胺的培养基中没有观察到明显的影响。这种缺失也会削弱 EPS 的合成,从而降低猪链球菌的生物膜形成能力。此外,研究还发现,luxS 基因缺失导致的粘附基因表达下调与 S. suis 的单糖介质无关。
期刊介绍:
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