An electrochemical biosensor to detect anti-asparaginase antibodies using immobilized ASNase on carbon-printed electrodes

IF 2.2 4区化学 Q2 Engineering

Chemical Papers Pub Date : 2024-09-17 DOI:10.1007/s11696-024-03681-2

Jocimara C. da Silva, Bruna Bragantin, Augusto Etchegaray, Jose A. Yunes, Ruchi Gupta, Renata K. Mendes

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Abstract

Acute lymphoblastic leukaemia (ALL) is one of the most common types of childhood cancer. Treatment of ALL may involve using the enzyme L-asparaginase (ASNase) from Escherichia coli as the first option. However, native E. coli ASNase can induce the production of antibodies, interfering with its pharmacological activity and increasing the risk of allergic reactions. Therefore, monitoring the production of anti-ASNase antibodies in patients is essential for determining treatment efficacy. Hence, a simple, precise, and selective method for the measurement of antibodies is of great interest. In this context, this work reports on an electrochemical biosensor based on carbon-printed electrodes to quantify antibodies in ALL patients under treatment using an ASNase-based protocol. For selective detection of anti-ASNase antibodies, ASNase was immobilized on electrodes using the nanocomposite Fe₃O₄/chitosan. The biosensor showed linearity for the antibody concentrations between 0.1 and 10 µg mL⁻¹, with a limit of detection of 150 ng L⁻¹. In addition, the biosensor was selective and provided reproducible results with a variability of 3.3%. From these results, it was concluded that the biosensor presented here is a promising device for the measurement of anti-ASNase antibodies produced in patients under treatment for ALL.

Graphical abstract

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利用固定在碳印电极上的 ASNase 检测抗天冬酰胺酶抗体的电化学生物传感器

急性淋巴细胞白血病（ALL）是最常见的儿童癌症之一。治疗急性淋巴细胞白血病的首选方法可能是使用大肠杆菌中的 L-天冬酰胺酶（ASNase）。然而，原生大肠杆菌 ASNase 可诱导抗体的产生，从而干扰其药理活性并增加过敏反应的风险。因此，监测患者体内抗 ASNase 抗体的产生对于确定疗效至关重要。因此，一种简单、精确且具有选择性的抗体测量方法备受关注。在此背景下，本研究报告了一种基于碳印刷电极的电化学生物传感器，用于定量正在接受基于 ASNase 方案治疗的 ALL 患者体内的抗体。为了选择性检测抗 ASNase 抗体，使用纳米复合材料 Fe3O4/ 壳聚糖将 ASNase 固定在电极上。该生物传感器在抗体浓度为 0.1 至 10 µg mL-1 之间呈线性关系，检测限为 150 ng L-1。此外，该生物传感器还具有选择性和可重复性，结果可变性仅为 3.3%。从这些结果中可以得出结论，本文介绍的生物传感器是一种很有前途的设备，可用于测量接受 ALL 治疗的患者体内产生的抗 ASNase 抗体。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemical Papers Chemical Engineering-General Chemical Engineering

CiteScore

3.30

自引率

4.50%

发文量

590

期刊介绍： Chemical Papers is a peer-reviewed, international journal devoted to basic and applied chemical research. It has a broad scope covering the chemical sciences, but favors interdisciplinary research and studies that bring chemistry together with other disciplines.