Detection of KPC-3 producing Escherichia coli ST410 in Volos, Greece.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-09-17 DOI:10.1556/030.2024.02376

Maria Chatzidimitriou,Pandora Tsolakidou,Maria Anna Kyriazidi,Stella Mitka

引用次数: 0

Abstract

Escherichia coli A382 was isolated in July 2024 from a positive blood culture obtained from the central venous catheter of a male patient undergoing chemotherapy at the Hospital of Volos, Thessaly, Greece. Whole-genome sequencing analysis revealed that the isolate A382 is E. coli belonging to the ST410 high-risk clone, which co-harbors the blaKPC-3 and blaSHV-182 genes on an IncX3 plasmid. It also harbors blaTEM-1 and has five replicons, as follows: IncX3, IncQ1, CoIRNAI, IncF1A, and IncFIB. Complete genome analysis revealed that E. coli A382 isolate carries a range of virulence factors (iutA, iucC, fimH, fdeC, yehA, yehD, yehC, yehB, cgs, ahha, ccI, hlyE, papC, irp2, fyuA, lpfA, and nlpl) and many other non-beta-lactam resistance determinants, including dfrA14 and sul2, but it is susceptible to aminoglycosides, nitrofurantoin, tigecycline, colistin and ceftazidime-avibactam. In conclusion in this study, we describe the phenotypic and genome characteristics of an extensively drug-resistant E. coli ST410.

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在希腊沃洛斯检测到产 KPC-3 的大肠杆菌 ST410。

2024 年 7 月，希腊塞萨利沃洛斯医院从一名正在接受化疗的男性患者的中心静脉导管中获得的阳性血液培养物中分离出大肠埃希菌 A382。全基因组测序分析表明，分离物 A382 是属于 ST410 高风险克隆的大肠杆菌，在一个 IncX3 质粒上共同携带 blaKPC-3 和 blaSHV-182 基因。它还携带 blaTEM-1，并有以下五个复制子：IncX3、IncQ1、CoIRNAI、IncF1A 和 IncFIB。全基因组分析表明大肠杆菌 A382 分离物携带一系列毒力因子（iutA、iucC、fimH、fdeC、yehA、yehD、yehC、yehB、ccs、ahha、ccI、hlyE、papC、irp2、fyuA、lpfA、和 nlpl）以及许多其他非β-内酰胺耐药基因，包括 dfrA14 和 sul2，但它对氨基糖苷类、硝基呋喃妥因、替加环素、大肠杆菌素和头孢唑肟-阿维巴坦易感。总之，本研究描述了一种广泛耐药大肠杆菌 ST410 的表型和基因组特征。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.