The influence of different radiotherapy doses on the mechanical properties and microstructure of the enamel and dentin of human premolar teeth

IF 2.7 3区医学 Q3 ONCOLOGY

Strahlentherapie und Onkologie Pub Date : 2024-09-16 DOI:10.1007/s00066-024-02296-6

Yuan Tu, Liying Hao, Yi Ding, Yisi Zhong, Chengge Hua, Li Jiang

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引用次数: 0

Abstract

Objective

Radiation therapy is applied in the treatment of head and neck cancer patients. However, oral-health-related side effects like hyposalivation and a higher prevalence of caries have been shown. This study aims to assess the influence of different radiotherapy doses on the mechanical properties, roughness, superficial microstructure, and crystallinity of the enamel and dentin of human premolar teeth.

Methods

Specimens (n = 25) were categorized into five groups based on the radiation dose received (0, 10, 30, 50, and 70 Gy). The enamel and dentin of these specimens were subjected to a microhardness tester, profilometer, atomic force microscopy (AFM), scanning electron microscopy (SEM), and X‑ray diffraction (XRD) before and after different irradiation doses and compared to hydroxylapatite in each group. The data were analyzed using repeated-measures analysis of variance (ANOVA).

Results

Therapeutic radiation doses of 30, 50, and 70 Gy led to a decrease in the microhardness and an increase in the average roughness of the enamel, and rougher surfaces were observed in the mixed three-dimensional images. Moreover, in the dentin, a similar outcome could be observed for more than 10 Gy. The main crystalline phase structure remained hydroxylapatite, but the crystallinity decreased and the crystalline size increased above 10 Gy. The superficial micromorphology revealed granulation, fissures, and cracks in a dose-dependent manner. Radiation below 70 Gy had little effect on the hydroxylapatite concentration during the whole experiment.

Conclusion

Above a radiation dose of 30 Gy, the micromorphology of the tooth enamel changed. This occurred for dentin above 10 Gy, which indicates that dentin is more sensitive to radiotherapy than enamel. The radiation dose had an effect on the micromorphology of the hard tissues of the teeth. These results illustrate the possible mechanism of radiation-related caries and have guiding significance for clinical radiotherapy.

Abstract Image

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不同放疗剂量对人类前臼齿牙釉质和牙本质的机械性能和微观结构的影响

目的放射治疗用于治疗头颈部癌症患者。然而，与口腔健康相关的副作用，如唾液分泌过少和龋齿发病率升高已被证实。本研究旨在评估不同放疗剂量对人类前臼齿牙釉质和牙本质的机械性能、粗糙度、表层微观结构和结晶度的影响。方法根据接受的放射剂量（0、10、30、50 和 70 Gy）将样本（n = 25）分为五组。在不同辐照剂量前后，对这些试样的珐琅质和牙本质进行显微硬度计、轮廓仪、原子力显微镜、扫描电子显微镜和 X 射线衍射，并与各组中的羟基磷灰石进行比较。结果治疗辐射剂量为 30、50 和 70 Gy 时，釉质的显微硬度下降，平均粗糙度增加，在混合三维图像中观察到更粗糙的表面。此外，在牙本质中，超过 10 Gy 也能观察到类似的结果。主要的结晶相结构仍然是羟基磷灰石，但超过 10 Gy 后结晶度降低，结晶尺寸增大。表层微观形态显示出肉芽、裂缝和裂纹，这与剂量有关。在整个实验过程中，低于 70 Gy 的辐射对羟基磷灰石浓度几乎没有影响。这表明牙本质比牙釉质对放射治疗更敏感。辐射剂量对牙齿硬组织的微观形态有影响。这些结果说明了辐射相关龋病的可能机制，对临床放射治疗具有指导意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Strahlentherapie und Onkologie 医学-核医学

CiteScore

5.70

自引率

12.90%

发文量

141

审稿时长

3-8 weeks

期刊介绍： Strahlentherapie und Onkologie, published monthly, is a scientific journal that covers all aspects of oncology with focus on radiooncology, radiation biology and radiation physics. The articles are not only of interest to radiooncologists but to all physicians interested in oncology, to radiation biologists and radiation physicists. The journal publishes original articles, review articles and case studies that are peer-reviewed. It includes scientific short communications as well as a literature review with annotated articles that inform the reader on new developments in the various disciplines concerned and hence allow for a sound overview on the latest results in radiooncology research. Founded in 1912, Strahlentherapie und Onkologie is the oldest oncological journal in the world. Today, contributions are published in English and German. All articles have English summaries and legends. The journal is the official publication of several scientific radiooncological societies and publishes the relevant communications of these societies.