In Vitro Probiotic Characterization of Yeasts with their Postbiotics' Antioxidant Activity and Biofilm Inhibition Capacity

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2024-09-17 DOI:10.1007/s00284-024-03881-9

Furkan Aydın, Yeşim Aktepe, Halil İbrahim Kahve, İbrahim Çakır

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Abstract

This study evaluated the in vitro probiotic potential and postbiotic properties of yeast strains isolated from traditional fermented foods, emphasizing antioxidant activity (AOA) and biofilm inhibition capacity (BIC). The yeasts were molecularly confirmed using start codon targeted polymorphisms as Kluyveromyces lactis (n = 17), Saccharomyces cerevisiae (n = 9), Pichia kudriavzevii (n = 6), P. fermentans (n = 4), Wickerhamomyces anomalus (n = 2), and Torulaspora delbrueckii (n = 1). The probiotic assessment of live cells included viability in simulated gastric and pancreatic juices, autoaggregation, hydrophobicity, and AOA, using S. boulardii MYA-796 as reference. Additionally, cell-free supernatants (CFS) were tested for AOA and BIC against Cronobacter sakazakii, Listeria monocytogenes, Pseudomonas aeruginosa, and Staphylococcus aureus. Several strains exhibited significantly higher in vitro probiotic characteristics compared to S. boulardii MYA-796 (P < 0.05), particularly in gastric and pancreatic survival, hydrophobicity, and AOA. Notably, CFS exhibited greater AOA than live cells and strong BIC, especially against L. monocytogenes and S. aureus. Multivariate analysis identified K. lactis TC11, S. cerevisiae M33T1-2, P. kudriavzevii S96, W. anomalus OB7Y1, and T. delbrueckii KY31 as having superior probiotic properties, attributed to enhanced gastric survival, autoaggregation, and AOA. CFS of S. cerevisiae M33T1-2 and T. delbrueckii KY31 demonstrated significant BIC, with over 60% inhibition across all tested pathogens.

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酵母的体外益生菌特性及其后生菌的抗氧化活性和生物膜抑制能力

本研究评估了从传统发酵食品中分离出来的酵母菌株的体外益生潜力和益生后特性，重点是抗氧化活性（AOA）和生物膜抑制能力（BIC）。利用起始密码子靶向多态性对酵母菌进行了分子鉴定，确认其为乳酸克鲁维酵母（Kluyveromyces lactis）（n = 17）、酿酒酵母（Saccharomyces cerevisiae）（n = 9）、Pichia kudriavzevii（n = 6）、P. fermentans（n = 4）、Wickerhamomyces anomalus（n = 2）和Torulaspora delbrueckii（n = 1）。活细胞益生菌评估包括在模拟胃液和胰液中的存活率、自聚集、疏水性和 AOA，以布拉氏酵母菌 MYA-796 为参考。此外，还测试了无细胞上清液（CFS）对阪崎克罗诺杆菌、李斯特菌、铜绿假单胞菌和金黄色葡萄球菌的 AOA 和 BIC。与布拉氏酵母菌 MYA-796 相比，一些菌株的体外益生特性明显更高（P < 0.05），特别是在胃和胰腺存活率、疏水性和 AOA 方面。值得注意的是，与活细胞相比，CFS 表现出更高的 AOA 和更强的 BIC，尤其是在抗单核细胞增多症和金黄色葡萄球菌方面。多变量分析表明，K. lactis TC11、S. cerevisiae M33T1-2、P. kudriavzevii S96、W. anomalus OB7Y1 和 T. delbrueckii KY31 具有更优越的益生特性，这归因于它们提高了胃存活率、自聚集和 AOA。S. cerevisiae M33T1-2 和 T. delbrueckii KY31 的 CFS 具有显著的 BIC，对所有测试病原体的抑制率超过 60%。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464