A tutorial on optical photothermal infrared (O-PTIR) microscopy

IF 5.4 1区物理与天体物理 Q1 OPTICS

APL Photonics Pub Date : 2024-09-13 DOI:10.1063/5.0219983

Craig B. Prater, Mustafa Kansiz, Ji-Xin Cheng

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Abstract

This tutorial reviews the rapidly growing field of optical photothermal infrared (O-PTIR) spectroscopy and chemical imaging. O-PTIR is an infrared super-resolution measurement technique where a shorter wavelength visible probe is used to measure and map infrared (IR) absorption with spatial resolution up to 30× better than conventional techniques such as Fourier transform infrared and direct IR laser imaging systems. This article reviews key limitations of conventional IR instruments, the O-PTIR technology breakthroughs, and their origins that have overcome the prior limitations. This article also discusses recent developments in expanding multi-modal O-PTIR approaches that enable complementary Raman spectroscopy and fluorescence microscopy imaging, including wide-field O-PTIR imaging with fluorescence-based detection of IR absorption. Various practical subjects are covered, including sample preparation techniques, optimal measurement configurations, use of IR tags/labels and techniques for data analysis, and visualization. Key O-PTIR applications are reviewed in many areas, including biological and biomedical sciences, environmental and microplastics research, (bio)pharmaceuticals, materials science, cultural heritage, forensics, photonics, and failure analysis.

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光学光热红外（O-PTIR）显微镜教程

本教程回顾了快速发展的光学光热红外（O-PTIR）光谱和化学成像领域。O-PTIR 是一种红外超分辨率测量技术，利用波长较短的可见光探头测量和绘制红外吸收图，其空间分辨率比傅立叶变换红外和直接红外激光成像系统等传统技术高出 30 倍。本文回顾了传统红外仪器的主要局限性、O-PTIR 技术的突破及其克服先前局限性的起源。本文还讨论了扩展多模态 O-PTIR 方法的最新进展，这些方法可实现互补的拉曼光谱和荧光显微成像，包括基于荧光检测红外吸收的宽视场 O-PTIR 成像。内容涵盖各种实用主题，包括样品制备技术、最佳测量配置、红外标记/标签的使用、数据分析技术和可视化。本书回顾了 O-PTIR 在许多领域的主要应用，包括生物和生物医学科学、环境和微塑料研究、（生物）制药、材料科学、文化遗产、法医、光子学和故障分析。

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来源期刊

APL Photonics Physics and Astronomy-Atomic and Molecular Physics, and Optics

CiteScore

10.30

自引率

3.60%

发文量

107

审稿时长

19 weeks

期刊介绍： APL Photonics is the new dedicated home for open access multidisciplinary research from and for the photonics community. The journal publishes fundamental and applied results that significantly advance the knowledge in photonics across physics, chemistry, biology and materials science.