Myonuclear position and blood vessel organization during skeletal muscle postnatal development.

IF 3.7 2区生物学 Q1 DEVELOPMENTAL BIOLOGY

Development Pub Date : 2024-09-17 DOI:10.1242/dev.202548

Catarina Sequeira,Lou Martha Wackerbarth,Andreia Pena,Mafalda Sá-Pereira,Cláudio A Franco,Edgar R Gomes

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Abstract

Skeletal muscle development is a complex process involving myoblast fusion to generate multinucleated fibers. Myonuclei first align in the center of the myotubes before migrating to the periphery of the myofiber. Blood vessels (BVs) are important contributors to the correct development of skeletal muscle, and myonuclei are found next to BVs in adult muscle. Here, we show that most myonuclear migration to the periphery occurs between E17.5 and P1. Furthermore, myonuclear accretion after P7 does not result in centrally nucleated myofibers as observed in the embryo. Instead, myonuclei remain at the periphery of the myofiber without moving to the center. Finally, we show that hypovascularization of skeletal muscle alters the interaction between myonuclei and BVs, suggesting that BVs may contribute to myonuclear positioning during skeletal muscle postnatal development. Overall, this work provides a comprehensive analysis of skeletal muscle development during the highly dynamic postnatal period, bringing new insights about myonuclear positioning and its interaction with BVs.

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骨骼肌产后发育过程中的肌核位置和血管组织

骨骼肌的发育是一个复杂的过程，涉及肌母细胞融合生成多核纤维。肌核首先在肌管中心排列，然后迁移到肌纤维外围。血管（BV）是骨骼肌正确发育的重要因素，而成肌中的肌核就在血管旁边。在这里，我们发现大部分肌核向外周的迁移发生在E17.5至P1期间。此外，P7 后的肌核增生并不像在胚胎中观察到的那样导致肌纤维向中心成核。相反，肌核停留在肌纤维外围，没有向中心移动。最后，我们发现骨骼肌的低血管化改变了肌核与 BV 之间的相互作用，这表明 BV 可能有助于骨骼肌产后发育过程中的肌核定位。总之，这项工作全面分析了骨骼肌在出生后高度动态时期的发育，为肌核定位及其与 BV 的相互作用带来了新的见解。

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来源期刊

Development 生物-发育生物学

CiteScore

6.70

自引率

4.30%

发文量

433

审稿时长

3 months

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