Transcriptomic Analysis of the Effects of Changes in Temperature and Flow Velocity on Reproductive Ability of Coreius guichenoti

IF 2.5 3区环境科学与生态学 Q2 ECOLOGY

Ecohydrology Pub Date : 2024-09-14 DOI:10.1002/eco.2721

Yuqing Lin, Ting Li, Qiuwen Chen, Ruxia Qiao, Shufeng He, Tao Feng, Jun Wang

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Abstract

The establishment of reservoirs, which alter water temperature and flow dynamics, significantly affects the indigenous fish population, yet the underlying molecular mechanisms remain unclear. To investigate effects of the changing water temperature and flow rate on fish ovaries, we subjected female Coreius guichenoti to the combination of different water temperatures (17°C, 20°C and 23°C denoted as L, N and H, respectively) and flow velocities (0 and 0.5 m/s denoted as S and V, respectively). After 30‐day experiment period, we identified 393, 545, 300 and 657 differentially expressed transcripts in HV versus NV, LV versus NV, HS versus NV and LS versus NV, respectively. The transcriptome results showed that both environmental factors could significantly influence oogenesis, with water temperature exerting a more pronounced effect than flow velocity. In particular, the higher temperature (HV and HS) led to a tendency towards masculinization and even infertility in females, which were consistent with the histopathology results. By contrast, the lower temperature (LV and LS) promoted the progression of desirable female attributes, where static water conditions (LS) had a greater effect compared with flowing water (LV). These findings were of great significance for the adaptive operation of reservoirs to create reasonable and precise ecological flows for managing fish reproduction.

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温度和流速变化对鲤鱼繁殖能力影响的转录组分析

水库的建立改变了水温和水流动态，对本地鱼类种群产生了重大影响，但其潜在的分子机制仍不清楚。为了研究水温和流速变化对鱼类卵巢的影响，我们将雌性Coreius guichenoti置于不同水温（分别为17°C、20°C和23°C，以L、N和H表示）和流速（分别为0和0.5 m/s，以S和V表示）的组合中。经过 30 天的实验，我们分别在 HV 与 NV、LV 与 NV、HS 与 NV 和 LS 与 NV 中发现了 393、545、300 和 657 个差异表达的转录本。转录组结果表明，两种环境因素都会显著影响卵子的发生，其中水温的影响比流速更明显。尤其是较高的温度（HV和HS）会导致雌鱼男性化甚至不育，这与组织病理学结果一致。相比之下，较低的温度（LV 和 LS）会促进雌性理想属性的发展，其中静水条件（LS）比流水条件（LV）的影响更大。这些发现对水库的适应性运行具有重要意义，可为管理鱼类繁殖创造合理、精确的生态流量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ecohydrology 环境科学-生态学

CiteScore

5.10

自引率

7.70%

发文量

116

审稿时长

24 months

期刊介绍： Ecohydrology is an international journal publishing original scientific and review papers that aim to improve understanding of processes at the interface between ecology and hydrology and associated applications related to environmental management. Ecohydrology seeks to increase interdisciplinary insights by placing particular emphasis on interactions and associated feedbacks in both space and time between ecological systems and the hydrological cycle. Research contributions are solicited from disciplines focusing on the physical, ecological, biological, biogeochemical, geomorphological, drainage basin, mathematical and methodological aspects of ecohydrology. Research in both terrestrial and aquatic systems is of interest provided it explicitly links ecological systems and the hydrologic cycle; research such as aquatic ecological, channel engineering, or ecological or hydrological modelling is less appropriate for the journal unless it specifically addresses the criteria above. Manuscripts describing individual case studies are of interest in cases where broader insights are discussed beyond site- and species-specific results.