Setting up an institutional OMERO environment for bioimage data: Perspectives from both facility staff and users

Abstract

Modern bioimaging core facilities at research institutions are essential for managing and maintaining high-end instruments, providing training and support for researchers in experimental design, image acquisition and data analysis. An important task for these facilities is the professional management of complex multidimensional bioimaging data, which are often produced in large quantity and very different file formats. This article details the process that led to successfully implementing the OME Remote Objects system (OMERO) for bioimage-specific research data management (RDM) at the Core Facility Cellular Imaging (CFCI) at the Technische Universität Dresden (TU Dresden). Ensuring compliance with the FAIR (findable, accessible, interoperable, reusable) principles, we outline here the challenges that we faced in adapting data handling and storage to a new RDM system. These challenges included the introduction of a standardised group-specific naming convention, metadata curation with tagging and Key–Value pairs, and integration of existing image processing workflows. By sharing our experiences, this article aims to provide insights and recommendations for both individual researchers and educational institutions intending to implement OMERO as a management system for bioimaging data. We showcase how tailored decisions and structured approaches lead to successful outcomes in RDM practices.

Lay description: Modern bioimaging facilities at research institutions are crucial for managing advanced equipment and supporting scientists in their research. These facilities help with designing experiments, capturing images, and analyzing data. One of their key tasks is organizing and managing large amounts of complex image data, which often comes in various file formats and are difficult to handle.

This article explains how the Core Facility Cellular Imaging (CFCI) at Technische Universität Dresden successfully implemented a specialized system called OMERO. With this system it is possible to manage and organize bioimaging data sustainably in a way that they are findable, accessible, interoperable and reusable according the FAIR principles. We describe the practical implementation process on exemplary projects within scientific research and medical education. We discuss the challenges we faced, such as creating a standard way to name files, organizing important information about the images (known as metadata), and ensuring that existing image processing methods could work with the new system.

By sharing our experience, we aim to offer practical advice and recommendations for other researchers and institutions interested in using OMERO for managing their bioimaging data. We highlight how careful planning and structured approaches can lead to successful data management practices, making it easier for researchers to store, access, and reuse their valuable data.