Elucidating light and temperature‐dependent signalling pathways from shoot to root in rice plants: Implications for stress responses

Abstract

The main aim of this work was to better understand how the low temperature signal from the leaves may affect the stress responses in the roots, and how the light conditions modify certain stress acclimation processes in rice plants. Rice plants grown at 27°C were exposed to low temperatures (12°C) with different light intensities, and in the case of some groups of plants, only the leaves received the cold, while the roots remained at control temperature. RNA sequencing focusing on the roots of plants grown under normal growth light conditions found 525 differentially expressed genes in different comparisons. Exposure to low temperature led to more down‐regulated than up‐regulated genes. Comparison between roots of the leaf‐stressed plants and whole cold‐treated or control plants revealed that nitrogen metabolism and nitric oxide‐related signalling, as well as the phenylpropanoid‐related processes, were specifically affected. Real‐time PCR results focusing on the COLD1 and polyamine oxidase genes, as well as metabolomics targeting hormonal changes and phenolic compounds also showed that not only cold exposure of the leaves, either alone or together with the roots, but also the light conditions may influence certain stress responses in the roots of rice plants.