Saeid Afzalinia, Mehdi Mehrabi-Koushki, Reza Farokhinejad
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引用次数: 0
Abstract
Identification of Fusarium species associated with diseases symptoms in plants is an important step toward understanding the ecology of plant–fungus associations. In this study, four Fusarium isolates were obtained from root rot of Oryza sativa L. in Izeh (southwest of Iran) and identified based on phylogenetic analyses combined with morphology. Phylogenetic analyses based on combined translation elongation factor 1-α, calmodulin, RNA polymerase II second largest subunit, and Beta-tubulin (tub2) sequence data delimited two new species, namely F. khuzestanicum and F. oryzicola spp. nov., from previously known species of Fusarium within F. incarnatum-equiseti species complex (FIESC). Morphologically, F. khuzestanicum produces the macroconidia with distinctly notched to foot-shaped basal cells, while basal cells in the macroconidia of F. oryzicola are more extended and distinctly elongated foot shape. Furthermore, these two new species are distinguished by the size of their sporodochial phialides and macroconidia. The results of the present show that the FIESC species complex represent more cryptic species.
鉴定与植物病害症状相关的镰刀菌种是了解植物与真菌关联生态学的重要一步。本研究从伊泽(伊朗西南部)Oryza sativa L.的根腐病中获得了四个镰刀菌分离株,并根据系统发育分析结合形态学进行了鉴定。基于翻译延伸因子 1-α、钙调蛋白、RNA 聚合酶 II 第二大亚基和 Beta-tubulin(tub2)序列数据的系统发育分析将两个新种,即 F. khuzestanicum 和 F. oryzicola spp.从形态上看,F. khuzestanicum 的大孢子器基部细胞明显呈缺刻状至足状,而 F. oryzicola 的大孢子器基部细胞则更长,呈明显的拉长足状。此外,这两个新种的区别还在于它们的孢子囊噬菌体和大锥体的大小。本研究结果表明,FIESC物种群代表了更多的隐生物种。
期刊介绍:
Antonie van Leeuwenhoek publishes papers on fundamental and applied aspects of microbiology. Topics of particular interest include: taxonomy, structure & development; biochemistry & molecular biology; physiology & metabolic studies; genetics; ecological studies; especially molecular ecology; marine microbiology; medical microbiology; molecular biological aspects of microbial pathogenesis and bioinformatics.