Genome-based taxonomy and functional prediction of Sphingomonas fuzhouensis sp. nov. and Massilia phyllosphaerae sp. nov. isolated from Pennisetum sp. with plant growth-promoting potential

IF 1.8 3区 生物学 Q4 MICROBIOLOGY
Ling Yao, Guo-Hong Liu, Shu-Yi Zhang, Peng Gao, Christopher Rensing, Qiu-E Yang, Shun-Gui Zhou
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Abstract

Two facultatively aerobic strains, designated SGZ-02T and SGZ-792T, were isolated from plant Pennisetum sp., exhibiting the highest 16S rRNA gene sequence similarities with the type strains of Sphingomonas zeae LMG 28739T (98.6%) and Massilia forsythiae NBRC 114511T (98.4%), respectively. SGZ-02T grew between 5 and 45 °C, pH 5.0–11.0 and tolerated NaCl concentrations of 0–4% (w/v), whereas SGZ-792T thrived at 5–40 °C, pH 5.0–11.0 and NaCl tolerance to 0–3.5% (w/v). The major quinone of SGZ-02T was ubiquinone-10, with the dominant fatty acids being C16:0 (13.5%), Summed Feature 3 (6.3%), C14:02-OH (5.3%) and Summed Feature 8 (66.3%). SGZ-792T predominantly contained ubiquinone-8, with major fatty acids being C16:0 (20.3%), Summed Feature 3 (5.0%) and Summed Feature 8 (54.7%). Average nucleotide identity and digital DNA–DNA hybridization values between two strains and their closest references strains were below the bacterial species threshold. Based on genotypic and phenotypic characteristics, strains SGZ-02T and SGZ-792T are proposed as novel species within the genera Sphingomonas and Massilia, respectively. The suggested names for the new species are Sphingomonas fuzhouensis sp. nov. (SGZ-02T = GDMCC 1.4033T = JCM 36769T) and Massilia phyllosphaerae sp. nov. (SGZ-792T = GDMCC 1.4211T = JCM 36643T), respectively.

Abstract Image

Abstract Image

基于基因组的福州单胞菌(Sphingomonas fuzhouensis sp.
从Pennisetum sp.植物中分离出两株兼性需氧菌株,命名为SGZ-02T和SGZ-792T,分别与Sphingomonas zeae LMG 28739T(98.6%)和Massilia forsythiae NBRC 114511T(98.4%)的模式菌株表现出最高的16S rRNA基因序列相似性。SGZ-02T 的生长温度为 5 至 45 °C,pH 值为 5.0 至 11.0,耐 NaCl 浓度为 0 至 4%(体积分数);而 SGZ-792T 的生长温度为 5 至 40 °C,pH 值为 5.0 至 11.0,耐 NaCl 浓度为 0 至 3.5%(体积分数)。SGZ-02T 的主要醌为泛醌-10,主要脂肪酸为 C16:0(13.5%)、特征总和 3(6.3%)、C14:02-OH(5.3%)和特征总和 8(66.3%)。SGZ-792T 主要含有泛醌-8,主要脂肪酸为 C16:0 (20.3%)、特征总和 3 (5.0%) 和特征总和 8 (54.7%)。两个菌株与其最接近的参考菌株之间的平均核苷酸同一性和数字 DNA-DNA 杂交值均低于细菌物种阈值。根据基因型和表型特征,建议将 SGZ-02T 和 SGZ-792T 菌株分别作为 Sphingomonas 属和 Massilia 属中的新种。新种的建议名称分别为福州单胞菌属新种(SGZ-02T = GDMCC 1.4033T = JCM 36769T)和马纤毛虫属新种(SGZ-792T = GDMCC 1.4211T = JCM 36643T)。
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来源期刊
CiteScore
5.60
自引率
11.50%
发文量
104
审稿时长
3 months
期刊介绍: Antonie van Leeuwenhoek publishes papers on fundamental and applied aspects of microbiology. Topics of particular interest include: taxonomy, structure & development; biochemistry & molecular biology; physiology & metabolic studies; genetics; ecological studies; especially molecular ecology; marine microbiology; medical microbiology; molecular biological aspects of microbial pathogenesis and bioinformatics.
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