Muhammad Saalim, Ya Lu, Kenneth J. Woycechowsky, Benjamin R. Clark
{"title":"A cheap and straightforward method for the selective isolation of histidine-derived natural products using nickel(II) phosphate","authors":"Muhammad Saalim, Ya Lu, Kenneth J. Woycechowsky, Benjamin R. Clark","doi":"10.1039/d4ay01467g","DOIUrl":null,"url":null,"abstract":"Nickel-based resins have long been used in biochemical studies for the purification of His-tagged proteins, but their potential in the isolation of histidine-derived small molecules has not been investigated to date. Many agriculturally-important mycotoxins incorporate histidine residues, as do natural products from both plants and bacteria. Here, a highly-selective solid-phase extraction method is described for the purification of histidine-derived natural products using the insoluble nickel salt Ni<small><sub>3</sub></small>(PO<small><sub>4</sub></small>)<small><sub>2</sub></small>. This led to the highly-selective binding and elution of two natural products, meleagrin and gartryprostatin C, from two fungal strains, <em>Penicillium chrysogenum</em> and <em>Aspergillus sclerotiorum</em>, respectively. A simple protocol involving binding, washing with water and methanol, and elution with methanolic acetate buffer, gave 65–75% recovery of both compounds directly from crude extracts of the two fungi. The procedure can easily be used as part of a multi-step isolation process in combination with HPLC to yield the purified alkaloids.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7000,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Methods","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1039/d4ay01467g","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Nickel-based resins have long been used in biochemical studies for the purification of His-tagged proteins, but their potential in the isolation of histidine-derived small molecules has not been investigated to date. Many agriculturally-important mycotoxins incorporate histidine residues, as do natural products from both plants and bacteria. Here, a highly-selective solid-phase extraction method is described for the purification of histidine-derived natural products using the insoluble nickel salt Ni3(PO4)2. This led to the highly-selective binding and elution of two natural products, meleagrin and gartryprostatin C, from two fungal strains, Penicillium chrysogenum and Aspergillus sclerotiorum, respectively. A simple protocol involving binding, washing with water and methanol, and elution with methanolic acetate buffer, gave 65–75% recovery of both compounds directly from crude extracts of the two fungi. The procedure can easily be used as part of a multi-step isolation process in combination with HPLC to yield the purified alkaloids.