Stage-Specific Biomarkers for Papaya Sticky Disease Using the Molecular Profile of Carica papaya (L.) Leaf Extracts: A Chromatographic Approach

IF 2.6 3区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Food Analytical Methods Pub Date : 2024-09-13 DOI:10.1007/s12161-024-02673-y

Isabella Oliveira Britto, Patricia Machado Bueno Fernandes, Alexandre Martins Costa Santos

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Abstract

Cultivated Carica papaya L. (Caricaceae) orchards are threatened by papaya sticky disease (PSD) caused by PMeV viral complex, leading to significant losses in fruit quality and production decline. Secondary metabolites with potential antiviral activity may serve as disease progression markers aiding in early diagnosis and chromatographic approach can assist in this detection. Here, the molecular profiles of crude extracts from C. papaya leaves in the initial stage of development and postflowering stages, with and without PSD symptoms, were evaluated using ultraviolet–visible spectroscopy, reverse-phase, and ion-exchange chromatography. Analytical parameters and chromatographic-based results indicate quantitatively higher chromatographic peaks in the initial stage leaf extract compared to postflowering leaf extracts, potentially reflecting the plant’s defense response against viral infection. This study aimed to identify biomarkers for PSD through the molecular profiling of C. papaya leaf extracts in different stages of the plant. Early disease detection using these biomarkers holds promise for reducing postharvest losses and ensuring fruit safety and quality for consumers allied to the existing diagnosis techniques.

Graphical Abstract

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利用木瓜（Carica papaya (L.)）叶提取物的分子谱分析木瓜粘病的阶段性特异性生物标志物：色谱法

栽培木瓜（Caricaceae）果园受到由 PMeV 病毒复合体引起的木瓜粘稠病（PSD）的威胁，导致果实质量严重受损，产量下降。具有潜在抗病毒活性的次生代谢物可作为疾病进展的标记物，有助于早期诊断，而色谱法可协助进行检测。本文采用紫外可见光谱法、反相色谱法和离子交换色谱法评估了木瓜叶片在发育初期和开花后阶段（有或无 PSD 症状）的粗提取物的分子特征。分析参数和基于色谱的结果表明，与开花后叶片提取物相比，初期叶片提取物的色谱峰定量更高，这可能反映了植物对病毒感染的防御反应。本研究旨在通过对木瓜不同阶段的叶片提取物进行分子谱分析，确定 PSD 的生物标志物。利用这些生物标志物进行早期病害检测有望减少采后损失，并与现有诊断技术相结合，确保消费者的水果安全和质量。图文摘要

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来源期刊

Food Analytical Methods 农林科学-食品科技

CiteScore

6.00

自引率

3.40%

发文量

244

审稿时长

3.1 months

期刊介绍： Food Analytical Methods publishes original articles, review articles, and notes on novel and/or state-of-the-art analytical methods or issues to be solved, as well as significant improvements or interesting applications to existing methods. These include analytical technology and methodology for food microbial contaminants, food chemistry and toxicology, food quality, food authenticity and food traceability. The journal covers fundamental and specific aspects of the development, optimization, and practical implementation in routine laboratories, and validation of food analytical methods for the monitoring of food safety and quality.