Biodegradable, biocompatible, and self-healing, injectable hydrogel based on oxidized Azadirachta indica gum and carboxymethyl chitosan through dynamic imine-linkage for biomedical application

IF 2.4 3区 化学 Q3 POLYMER SCIENCE
Jitendra Kumar, Roli Purwar
{"title":"Biodegradable, biocompatible, and self-healing, injectable hydrogel based on oxidized Azadirachta indica gum and carboxymethyl chitosan through dynamic imine-linkage for biomedical application","authors":"Jitendra Kumar, Roli Purwar","doi":"10.1007/s13726-024-01386-7","DOIUrl":null,"url":null,"abstract":"<p>At physiological temperature (37 °C), neem gum (NG) containing multialdehyde groups (NG-CHO) and carboxymethyl chitosan (CMCh) were combined to formulate an injectable hydrogel through the utilization of dynamic imine linkages. The investigation comprises determining the ideal oxidizing agent concentration to maximize the amount of aldehydes in neem gum. The best-oxidized neem gum determined the minimum gelation time (3 min). The injectable hydrogel's syringeability (~ 99%), porosity (47–71%), self-healing ability, and gel content (58–76%) were evaluated. Characterization techniques, including FTIR and <sup>1</sup>H NMR, confirmed the synthesis, and FESEM showed the morphology of the injectable hydrogel (pore size ~ 80 to 120 µm). Rheometry technique was used for quantitative analysis of shear-thinning behavior and self-healing ability. The swelling ratio was assessed for dry and gel forms of injectable hydrogels in phosphate buffer saline (PBS) at pH 5.5, 7.4, and 8.5. The swelling ratio decreased as the pH increased (5.5–7.4) and then increased at pH 8.5 for all synthesized injectable hydrogels (IHs) in dry and gel forms. For the release assay, an antibiotic model drug, ciprofloxacin HCl (Cipro), was loaded in situ, and the drug release behavior of dry and gel forms of injectable hydrogel was compared within all pH ranges. The drug release kinetics were estimated using the Korsmeyer–Peppas model. Cytotoxicity evaluation using L-929 fibroblast cell lines, antibacterial assay, and hydrolytic degradability (20–65%) in phosphate buffer saline at pH 7.4 was also conducted.</p><h3 data-test=\"abstract-sub-heading\">Graphical Abstract</h3>\n","PeriodicalId":601,"journal":{"name":"Iranian Polymer Journal","volume":null,"pages":null},"PeriodicalIF":2.4000,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Polymer Journal","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1007/s13726-024-01386-7","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"POLYMER SCIENCE","Score":null,"Total":0}
引用次数: 0

Abstract

At physiological temperature (37 °C), neem gum (NG) containing multialdehyde groups (NG-CHO) and carboxymethyl chitosan (CMCh) were combined to formulate an injectable hydrogel through the utilization of dynamic imine linkages. The investigation comprises determining the ideal oxidizing agent concentration to maximize the amount of aldehydes in neem gum. The best-oxidized neem gum determined the minimum gelation time (3 min). The injectable hydrogel's syringeability (~ 99%), porosity (47–71%), self-healing ability, and gel content (58–76%) were evaluated. Characterization techniques, including FTIR and 1H NMR, confirmed the synthesis, and FESEM showed the morphology of the injectable hydrogel (pore size ~ 80 to 120 µm). Rheometry technique was used for quantitative analysis of shear-thinning behavior and self-healing ability. The swelling ratio was assessed for dry and gel forms of injectable hydrogels in phosphate buffer saline (PBS) at pH 5.5, 7.4, and 8.5. The swelling ratio decreased as the pH increased (5.5–7.4) and then increased at pH 8.5 for all synthesized injectable hydrogels (IHs) in dry and gel forms. For the release assay, an antibiotic model drug, ciprofloxacin HCl (Cipro), was loaded in situ, and the drug release behavior of dry and gel forms of injectable hydrogel was compared within all pH ranges. The drug release kinetics were estimated using the Korsmeyer–Peppas model. Cytotoxicity evaluation using L-929 fibroblast cell lines, antibacterial assay, and hydrolytic degradability (20–65%) in phosphate buffer saline at pH 7.4 was also conducted.

Graphical Abstract

Abstract Image

基于氧化杜鹃胶和羧甲基壳聚糖的可生物降解、生物相容性和自愈合注射用水凝胶,通过动态亚胺连接实现生物医学应用
在生理温度(37 °C)下,将含有多醛基团的印楝胶(NG)(NG-CHO)和羧甲基壳聚糖(CMCh)结合起来,通过利用动态亚胺连接配制出一种可注射的水凝胶。这项研究包括确定理想的氧化剂浓度,以最大限度地增加楝树胶中的醛含量。氧化效果最佳的楝树胶决定了凝胶时间最短(3 分钟)。对可注射水凝胶的可注射性(约 99%)、孔隙率(47-71%)、自愈合能力和凝胶含量(58-76%)进行了评估。傅立叶变换红外光谱(FTIR)和 1H NMR 等表征技术证实了合成过程,而 FESEM 则显示了可注射水凝胶的形态(孔径约为 80 至 120 µm)。流变仪技术用于定量分析剪切稀化行为和自愈合能力。在 pH 值为 5.5、7.4 和 8.5 的磷酸盐缓冲盐水(PBS)中,对干型和凝胶型注射用水凝胶的溶胀率进行了评估。所有合成的干型和凝胶型可注射水凝胶(IHs)的溶胀率都随着 pH 值的升高(5.5-7.4)而降低,然后在 pH 值为 8.5 时升高。在释放试验中,原位负载了抗生素模型药物盐酸环丙沙星(Cipro),并比较了干型和凝胶型可注射水凝胶在所有 pH 值范围内的药物释放行为。药物释放动力学采用 Korsmeyer-Peppas 模型进行估计。此外,还利用 L-929 成纤维细胞系进行了细胞毒性评估、抗菌试验,并在 pH 值为 7.4 的磷酸盐缓冲盐水中进行了水解降解(20-65%)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Iranian Polymer Journal
Iranian Polymer Journal 化学-高分子科学
CiteScore
4.90
自引率
9.70%
发文量
107
审稿时长
2.8 months
期刊介绍: Iranian Polymer Journal, a monthly peer-reviewed international journal, provides a continuous forum for the dissemination of the original research and latest advances made in science and technology of polymers, covering diverse areas of polymer synthesis, characterization, polymer physics, rubber, plastics and composites, processing and engineering, biopolymers, drug delivery systems and natural polymers to meet specific applications. Also contributions from nano-related fields are regarded especially important for its versatility in modern scientific development.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信