Quantification of morphine in exhaled breath condensate using a double network polymeric hybrid hydrogel functionalized with AuNPs

IF 4.3 2区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

BMC Chemistry Pub Date : 2024-09-18 DOI:10.1186/s13065-024-01299-9

Zahra Karimzadeh, Abolghasem Jouyban, Maryam Khoubnasabjafari, Vahid Jouyban-Gharamaleki, Elaheh Rahimpour

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引用次数: 0

Abstract

Background

Morphine serves as a foundation for creating other opioid derivatives, such as hydro/oxymorphine and heroin, which possess enhanced pain-relieving properties but are also prone to addiction and abuse. In cases of morphine overdose, it not only affects multiple immune functions but can also cause severe health complications. Given these concerns and the widespread use of morphine, it is crucial to develop efficient, uncomplicated, and precise methods for accurately detecting morphine in various biological and pharmaceutical samples.

Results

In this investigation, a novel gold nanoparticle (AuNPs)-based double network hydrogel (DNH) nanoprobe has been fabricated for sensitive quantification of morphine in exhaled breath condensate samples. For that, gelatin/agarose DNH was fabricated through a one-step heating-cooling method in the presence of AuNPs, providing not only chemical stability but also prevent the AuNPs aggregation during synthesis process. In this method, the absorbance intensity of the nanoprobe gradually decreased with increasing morphine concentration due to the interaction morphine with AuNPs surface plasmon. The aggregation of AuNPs by addition of morphine was verified by UV-Vis spectrophotometry. The sensor displayed high sensitivity with detection limit of 0.006 µg.mL^-1 in the linear range from 0.01 to 1.0 µg.mL^-1. A reliable performance was attained for the spectrophotometric method for determination of morphine in the real samples.

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用 AuNPs 功能化的双网络聚合物杂化水凝胶定量检测呼出气体冷凝物中的吗啡含量

吗啡是制造其他阿片类衍生物（如氢/氧吗啡和海洛因）的基础，这些衍生物具有更强的止痛特性，但也容易成瘾和滥用。在吗啡过量的情况下，它不仅会影响多种免疫功能，还会引起严重的健康并发症。鉴于这些问题和吗啡的广泛使用，开发高效、简便、精确的方法来准确检测各种生物和药物样本中的吗啡至关重要。本研究制作了一种新型的基于金纳米粒子（AuNPs）的双网络水凝胶（DNH）纳米探针，用于对呼出的呼气冷凝物样品中的吗啡进行灵敏定量。明胶/琼脂糖 DNH 是在 AuNPs 存在下通过一步加热-冷却方法制成的，不仅具有化学稳定性，还能防止 AuNPs 在合成过程中聚集。在这种方法中，由于吗啡与 AuNPs 表面等离子体的相互作用，纳米探针的吸光度随着吗啡浓度的增加而逐渐降低。紫外可见分光光度法验证了加入吗啡后 AuNPs 的聚集情况。该传感器灵敏度高，在 0.01 至 1.0 µg.mL-1 的线性范围内，检测限为 0.006 µg.mL-1。分光光度法测定实际样品中吗啡的性能可靠。本文报告了一种基于双网络聚合物杂化水凝胶的新型先进光学支架，用于吗啡的测定。这是首次报道使用这种复合材料测定呼出气体冷凝物样品中的阿片类物质。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Chemistry Chemistry-General Chemistry

CiteScore

5.30

自引率

2.20%

发文量

审稿时长

27 weeks

期刊介绍： BMC Chemistry, formerly known as Chemistry Central Journal, is now part of the BMC series journals family. Chemistry Central Journal has served the chemistry community as a trusted open access resource for more than 10 years – and we are delighted to announce the next step on its journey. In January 2019 the journal has been renamed BMC Chemistry and now strengthens the BMC series footprint in the physical sciences by publishing quality articles and by pushing the boundaries of open chemistry.