In vitro anticancer efficacy of Calendula Officinalis extract-loaded chitosan nanoparticles against gastric and colon cancer cells.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2024-09-13 DOI:10.1080/03639045.2024.2404143

Rabia Yilmaz Ozturk,Rabia Cakir

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引用次数: 0

Abstract

OBJECTIVE This study assessed the anticancer activities of calendula officinalis-loaded chitosan nanoparticles in gastric and colon cancer cells compared to fibroblast cells and examined the balance between ROS and antioxidants. METHODS Considering this information, we synthesized Calendula officinalis-loaded chitosan nanoparticles (CO-CSNPs) via the ionic gelation method. Their characterizations were carried out with ZetaSizer, UV-Vis, FTIR and SEM devices including size, morphology and surface zeta potential analysis, loading capacity, encapsulation efficiency, in vitro drug release, and chemical interactions. The anticancer activities of CO, CSNPs, and CO-CSNPs were tested against AGS, Caco-2, and normal NIH-3T3 cells using an XTT assay. The anticancer effects were evaluated with the DAPI staining, scratch assay, reactive oxygen species (ROS) detection and CUPRAC method on cellular and non-cellular processes that promote anticancer mechanisms. RESULTS Results showed that CO and CO-CNPs exhibited anticancer activity against AGS and Caco-2. Further, the formulation of CO with CSNPs enhanced the anticancer activity of CO while having no cytotoxicity on NIH-3T3. DAPI staining, scratch assay, ROS, and CUPRAC method confirmed the anticancer activity of CO and CO-CSNPs, which resulted in a reduction in the number of apoptotic cells, inhibited migration, triggered apoptotic pathway via ROS, and higher antioxidant activity. CONCLUSIONS The results of the study indicate that CO-CSNPs are a promising therapeutic formulation for gastric and colon cancer treatment. We consider that this study will lead to the investigation of molecular mechanisms of CO-CSNPs in cancer treatment and their investigation in clinical studies.

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金盏花提取物负载壳聚糖纳米粒子对胃癌和结肠癌细胞的体外抗癌功效

目的本研究评估了金盏花壳聚糖纳米颗粒在胃癌和结肠癌细胞中与成纤维细胞相比的抗癌活性，并考察了 ROS 与抗氧化剂之间的平衡。方法考虑到这些信息，我们通过离子凝胶法合成了金盏花壳聚糖纳米颗粒（CO-CSNPs）。利用 ZetaSizer、UV-Vis、FTIR 和 SEM 设备对其进行了表征，包括尺寸、形态和表面 zeta 电位分析、负载能力、包封效率、体外药物释放和化学相互作用。使用 XTT 法测试了 CO、CSNPs 和 CO-CSNPs 对 AGS、Caco-2 和正常 NIH-3T3 细胞的抗癌活性。结果表明，CO 和 CO-CNPs 对 AGS 和 Caco-2 具有抗癌活性。此外，CO 与 CSNPs 的配制增强了 CO 的抗癌活性，但对 NIH-3T3 无细胞毒性。DAPI 染色、划痕试验、ROS 和 CUPRAC 法证实了 CO 和 CO-CSNPs 的抗癌活性，其结果是减少了凋亡细胞的数量，抑制了迁移，通过 ROS 触发了细胞凋亡途径，并提高了抗氧化活性。我们认为这项研究将有助于研究 CO-CSNPs 治疗癌症的分子机制，并将其应用于临床研究。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464