{"title":"An AIE-based ratiometric fluorescent probe for highly selective detection of H2S in plant stress responses","authors":"","doi":"10.1016/j.bios.2024.116798","DOIUrl":null,"url":null,"abstract":"<div><div>Hydrogen sulfide (H<sub>2</sub>S) has emerged as a crucial signaling molecule in plant stress responses, playing a significant role in regulating various physiological and biochemical processes. In this study, we report an aggregation-induced emission (AIE)-based ratiometric fluorescent probe <strong>TPN-H</strong><sub><strong>2</strong></sub><strong>S</strong> for the highly selective detection of H<sub>2</sub>S in plant tissues. The probe exhibited excellent sensitivity and selectivity towards H<sub>2</sub>S over other analytes, enabling real-time monitoring of H<sub>2</sub>S dynamics in living cell. Furthermore, the AIE-based ratiometric probe <strong>TPN-H</strong><sub><strong>2</strong></sub><strong>S</strong> allowed for accurate quantification of H<sub>2</sub>S levels, providing valuable insights into the spatiotemporal distribution of Cys metabolism produces H<sub>2</sub>S. Importantly, the physiological pathways and signaling mechanisms of H<sub>2</sub>S production of was investigated in plant tissues under Cr and nano-plastics stress. Utilizing a high-throughput screening approach, we identified exogenous substances such as calcium chloride (CaCl<sub>2</sub>) and abscisic acid (ABA) that could induce higher level of H<sub>2</sub>S production during the stress response in plants. Overall, those findings demonstrate the potential of the AIE-based ratiometric fluorescent probe <strong>TPN-H</strong><sub><strong>2</strong></sub><strong>S</strong> as a powerful tool for unraveling the role of H<sub>2</sub>S in plant stress responses and pave the way for further exploration of H<sub>2</sub>S-mediated signaling pathways in plants.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7000,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biosensors and Bioelectronics","FirstCategoryId":"1","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0956566324008042","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOPHYSICS","Score":null,"Total":0}
引用次数: 0
Abstract
Hydrogen sulfide (H2S) has emerged as a crucial signaling molecule in plant stress responses, playing a significant role in regulating various physiological and biochemical processes. In this study, we report an aggregation-induced emission (AIE)-based ratiometric fluorescent probe TPN-H2S for the highly selective detection of H2S in plant tissues. The probe exhibited excellent sensitivity and selectivity towards H2S over other analytes, enabling real-time monitoring of H2S dynamics in living cell. Furthermore, the AIE-based ratiometric probe TPN-H2S allowed for accurate quantification of H2S levels, providing valuable insights into the spatiotemporal distribution of Cys metabolism produces H2S. Importantly, the physiological pathways and signaling mechanisms of H2S production of was investigated in plant tissues under Cr and nano-plastics stress. Utilizing a high-throughput screening approach, we identified exogenous substances such as calcium chloride (CaCl2) and abscisic acid (ABA) that could induce higher level of H2S production during the stress response in plants. Overall, those findings demonstrate the potential of the AIE-based ratiometric fluorescent probe TPN-H2S as a powerful tool for unraveling the role of H2S in plant stress responses and pave the way for further exploration of H2S-mediated signaling pathways in plants.
期刊介绍:
Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.