E5 treatment showing improved health-span and lifespan in old Sprague Dawley rats

IF 8 1区 医学 Q1 CELL BIOLOGY
Aging Cell Pub Date : 2024-09-19 DOI:10.1111/acel.14335
Kavita Singh, Shraddha I. Khairnar, Akshay Sanghavi, Tanuja T. Yadav, Neha Gupta, Jay Arora, Harold L. Katcher
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Abstract

Aging and, in particular, the emergence of age-related disorders is associated with tissue dysfunction and macromolecular damage, some of which can be attributable to accumulated oxidative damage. In the current study, we determine the potential of ‘plasma-derived fraction (E5)’ for cellular rejuvenation and extending the lifespan of Sprague Dawley (SD) rats. This is a unique study wherein we have used 24-month-old rats and monitored them until the end of their lifespan with and without E5 treatment. In the present investigation, the SD rats were separated into two groups old control group and the treatment group (n = 8). The treatment group received four injections of E5 every alternate day for 8 days, and eight injections every alternate day for 16 days. Body weight, grip strength, cytokines, and biochemical markers were measured for more than 400 days of the study. Clinical observation, necropsy, and histology were performed. The E5 treatment exhibited great potential by showing significantly improved grip strength, remarkably decreased pro-inflammatory markers of chronic inflammation and oxidative stress, as well as biomarkers for vital organs (BUN, SGPT, SGOT, and triglycerides), and increased anti-oxidant levels. Clinical examinations, necropsies, and histopathology revealed that the animals treated with the E5 had normal cellular structure and architecture. In conclusion, this unique ‘plasma-derived exosome’ treatment (E5) alone is adequate to improve the health-span and extend the lifespan of the old SD rats significantly.

Abstract Image

E5 治疗显示老龄 Sprague Dawley 大鼠的健康寿命和寿命得到改善
衰老,尤其是老年性疾病的出现与组织功能障碍和大分子损伤有关,其中一些可归因于累积的氧化损伤。在目前的研究中,我们确定了 "血浆衍生成分(E5)"在细胞年轻化和延长斯普拉格道利(SD)大鼠寿命方面的潜力。这是一项独特的研究,我们使用了 24 个月大的大鼠,并对它们进行了监测,直到它们的寿命结束时,对它们进行了 E5 处理或未进行 E5 处理。在本次研究中,SD 大鼠被分为两组,即老对照组和治疗组(n = 8)。对照组每隔一天注射四次E5,共注射8天;治疗组每隔一天注射八次E5,共注射16天。在超过 400 天的研究期间,对体重、握力、细胞因子和生化指标进行了测量。还进行了临床观察、尸体解剖和组织学检查。E5 治疗显示出巨大的潜力,它显著改善了握力,明显降低了慢性炎症和氧化应激的前炎症标志物以及重要器官的生物标志物(BUN、SGPT、SGOT 和甘油三酯),并提高了抗氧化剂水平。临床检查、尸体解剖和组织病理学显示,接受 E5 治疗的动物细胞结构和构造正常。总之,仅用这种独特的 "血浆外泌体 "疗法(E5)就足以明显改善老龄 SD 大鼠的健康寿命并延长其寿命。
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来源期刊
Aging Cell
Aging Cell Biochemistry, Genetics and Molecular Biology-Cell Biology
自引率
2.60%
发文量
212
期刊介绍: Aging Cell is an Open Access journal that focuses on the core aspects of the biology of aging, encompassing the entire spectrum of geroscience. The journal's content is dedicated to publishing research that uncovers the mechanisms behind the aging process and explores the connections between aging and various age-related diseases. This journal aims to provide a comprehensive understanding of the biological underpinnings of aging and its implications for human health. The journal is widely recognized and its content is abstracted and indexed by numerous databases and services, which facilitates its accessibility and impact in the scientific community. These include: Academic Search (EBSCO Publishing) Academic Search Alumni Edition (EBSCO Publishing) Academic Search Premier (EBSCO Publishing) Biological Science Database (ProQuest) CAS: Chemical Abstracts Service (ACS) Embase (Elsevier) InfoTrac (GALE Cengage) Ingenta Select ISI Alerting Services Journal Citation Reports/Science Edition (Clarivate Analytics) MEDLINE/PubMed (NLM) Natural Science Collection (ProQuest) PubMed Dietary Supplement Subset (NLM) Science Citation Index Expanded (Clarivate Analytics) SciTech Premium Collection (ProQuest) Web of Science (Clarivate Analytics) Being indexed in these databases ensures that the research published in Aging Cell is discoverable by researchers, clinicians, and other professionals interested in the field of aging and its associated health issues. This broad coverage helps to disseminate the journal's findings and contributes to the advancement of knowledge in geroscience.
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