The Ratio of A400/A1800 Mapping Identifies Chromosomal Regions Containing Known Photoprotection Recovery-Related Genes in Rice

IF 4.8 1区农林科学 Q1 AGRONOMY

Rice Pub Date : 2024-09-16 DOI:10.1186/s12284-024-00739-3

Shoumik Saha, Nilanjan Sinha Mahapatra, Kriti Bhattacharya, Rimpa Kundu, K. Nimitha, Shamba Ganguly, Sebantee Ganguly, Tirthankar Biswas, Prabir K Bhattacharyya, Somnath Bhattacharyya

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Abstract

The rice, like other plants, undergoes photoprotection mode by increasing nonphotochemical quenching (NPQ) in high light intensity (> 1200 µmol m^− 2s^− 1 PPFD), which attenuates photosystem II yield (φPSII) drastically. The plant remains in photoprotection mode even after light intensity becomes not stressful for an extended period. While there are significant differences in the time it takes for photoprotection to recover among different genotypes, its use is limited in plant breeding because measuring the chlorophyll fluorescence parameters in progressive actinic light after dark adaptation takes more than forty-five minutes per genotype. The study finds that instantly measured A₄₀₀/A₁₈₀₀ ratio by five minutes in flag leaves of 25 diverse genotypes strongly associated with the φPSII₄₀₀ differences between theoretical and actual, qPd₄₀₀ and NPQ₄₀₀ with R² values 0.74, 0.65 and 0.60, respectively. In two consecutive years, GWAS of A₄₀₀/A₁₈₀₀ ratio identified the regions with genes reported earlier for plant photoprotection recovery. Additionally, QTL analysis in a RIL population also identified the regions carrying known genes related to photoprotection. Thus, the A₄₀₀/A₁₈₀₀ ratio can quickly phenotype many plants for easier introgression of the traits in popular cultivars. The identified genotypes, genes, and QTLs can be used to improve yield potential and allele mining.

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A400/A1800 映射比确定了含有已知光保护恢复相关基因的水稻染色体区域

水稻和其他植物一样，在高光照强度（1200 µmol m- 2s- 1 PPFD）下通过增加非光化学淬灭（NPQ）来实现光保护模式，从而大幅降低光系统 II 的产量（φPSII）。即使光照强度在相当长的一段时间内不再对植物产生压力，植物仍然处于光保护模式。虽然不同基因型光保护恢复所需的时间存在显著差异，但其在植物育种中的应用却很有限，因为测量暗适应后渐进式光照下的叶绿素荧光参数，每个基因型都需要四十五分钟以上的时间。研究发现，在 25 个不同基因型的旗叶中，5 分钟内即时测量的 A400/A1800 比率与φPSII400 理论值与实际值的差异、qPd400 和 NPQ400 密切相关，R2 值分别为 0.74、0.65 和 0.60。连续两年，A400/A1800 比率的 GWAS 发现了早先报道的植物光保护恢复基因区域。此外，在一个 RIL 群体中进行的 QTL 分析也确定了携带光保护相关已知基因的区域。因此，A400/A1800 比率可以快速对许多植物进行表型分析，从而更容易地将这些性状引入流行的栽培品种中。确定的基因型、基因和 QTLs 可用于提高产量潜力和等位基因挖掘。

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来源期刊

Rice AGRONOMY-

CiteScore

10.10

自引率

3.60%

发文量

审稿时长

>12 weeks

期刊介绍： Rice aims to fill a glaring void in basic and applied plant science journal publishing. This journal is the world''s only high-quality serial publication for reporting current advances in rice genetics, structural and functional genomics, comparative genomics, molecular biology and physiology, molecular breeding and comparative biology. Rice welcomes review articles and original papers in all of the aforementioned areas and serves as the primary source of newly published information for researchers and students in rice and related research.