{"title":"The Ratio of A400/A1800 Mapping Identifies Chromosomal Regions Containing Known Photoprotection Recovery-Related Genes in Rice","authors":"Shoumik Saha, Nilanjan Sinha Mahapatra, Kriti Bhattacharya, Rimpa Kundu, K. Nimitha, Shamba Ganguly, Sebantee Ganguly, Tirthankar Biswas, Prabir K Bhattacharyya, Somnath Bhattacharyya","doi":"10.1186/s12284-024-00739-3","DOIUrl":null,"url":null,"abstract":"<p>The rice, like other plants, undergoes photoprotection mode by increasing nonphotochemical quenching (NPQ) in high light intensity (> 1200 µmol m<sup>− 2</sup>s<sup>− 1</sup> PPFD), which attenuates photosystem II yield (φPSII) drastically. The plant remains in photoprotection mode even after light intensity becomes not stressful for an extended period. While there are significant differences in the time it takes for photoprotection to recover among different genotypes, its use is limited in plant breeding because measuring the chlorophyll fluorescence parameters in progressive actinic light after dark adaptation takes more than forty-five minutes per genotype. The study finds that instantly measured A<sub>400</sub>/A<sub>1800</sub> ratio by five minutes in flag leaves of 25 diverse genotypes strongly associated with the φPSII<sub>400</sub> differences between theoretical and actual, qPd<sub>400</sub> and NPQ<sub>400</sub> with R<sup>2</sup> values 0.74, 0.65 and 0.60, respectively. In two consecutive years, GWAS of A<sub>400</sub>/A<sub>1800</sub> ratio identified the regions with genes reported earlier for plant photoprotection recovery. Additionally, QTL analysis in a RIL population also identified the regions carrying known genes related to photoprotection. Thus, the A<sub>400</sub>/A<sub>1800</sub> ratio can quickly phenotype many plants for easier introgression of the traits in popular cultivars. The identified genotypes, genes, and QTLs can be used to improve yield potential and allele mining.</p>","PeriodicalId":21408,"journal":{"name":"Rice","volume":null,"pages":null},"PeriodicalIF":4.8000,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Rice","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1186/s12284-024-00739-3","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0
Abstract
The rice, like other plants, undergoes photoprotection mode by increasing nonphotochemical quenching (NPQ) in high light intensity (> 1200 µmol m− 2s− 1 PPFD), which attenuates photosystem II yield (φPSII) drastically. The plant remains in photoprotection mode even after light intensity becomes not stressful for an extended period. While there are significant differences in the time it takes for photoprotection to recover among different genotypes, its use is limited in plant breeding because measuring the chlorophyll fluorescence parameters in progressive actinic light after dark adaptation takes more than forty-five minutes per genotype. The study finds that instantly measured A400/A1800 ratio by five minutes in flag leaves of 25 diverse genotypes strongly associated with the φPSII400 differences between theoretical and actual, qPd400 and NPQ400 with R2 values 0.74, 0.65 and 0.60, respectively. In two consecutive years, GWAS of A400/A1800 ratio identified the regions with genes reported earlier for plant photoprotection recovery. Additionally, QTL analysis in a RIL population also identified the regions carrying known genes related to photoprotection. Thus, the A400/A1800 ratio can quickly phenotype many plants for easier introgression of the traits in popular cultivars. The identified genotypes, genes, and QTLs can be used to improve yield potential and allele mining.
期刊介绍:
Rice aims to fill a glaring void in basic and applied plant science journal publishing. This journal is the world''s only high-quality serial publication for reporting current advances in rice genetics, structural and functional genomics, comparative genomics, molecular biology and physiology, molecular breeding and comparative biology. Rice welcomes review articles and original papers in all of the aforementioned areas and serves as the primary source of newly published information for researchers and students in rice and related research.