A Liquid Crystal-based Biomaterial Platform for Rapid Sensing of Heat Stress using Machine learning

Abstract

Novel biomaterials that bridge the knowledge gap in coupling molecular/protein signatures of disease/stress via rapid readouts is a critical need of society. One such scenario is an imbalance between bodily heat production and heat dissipation which leads to heat stress in organisms. In addition to diminished animal well-being, heat stress is detrimental to the poultry industry as poultry entails fast growth and high yield, resulting in greater metabolic activity and higher body heat produc-tion. When stressed, cells overexpress heat shock proteins (such as HSP70, a well-established intracellular stress indicator) and may undergo changes in their mechanical properties. Liquid crystals (LCs, fluids with orientational order) are facile sen-sors as they can readily transduce chemical signals to easily observable optical responses. In this work, we introduce a hy-brid LC-cell biomaterial within which the difference in the expression of HSP70 is linked to optical changes in the response pattern via the use of convolutional neural networks (CNNs). The machine-learning (ML) models were trained on hundreds of such LC-response micrographs of chicken red blood cells with and without heat stress. Trained models exhibited remark-able accuracy of up to 99% on detecting the presence of heat stress in unseen microscope samples. We also show that cross-linking the chicken and human RBCs using glutaraldehyde in order to simulate a diseased cell was an efficient strategy for planning, building, training, and evaluating ML models. Overall, our efforts build towards desgining biomaterials that can rapidly detect disease in organisms that is accompanied by a distinct change in the mechanical properties of cells. We aim to eventuate CNN-enabled LC-sensors can rapidly report the presence of disease in scenarios where human judgment could be prohibitively difficult or slow.