Feida Che, Xiaoming Zhao, Qi Ding, Xiwei Li, Wen Zhang, Ping Li, Xin Wang and Bo Tang
{"title":"A versatile fluorescent probe for hydrogen peroxide in serotonergic neurons of living brains of mice with depression†","authors":"Feida Che, Xiaoming Zhao, Qi Ding, Xiwei Li, Wen Zhang, Ping Li, Xin Wang and Bo Tang","doi":"10.1039/D4TB01828A","DOIUrl":null,"url":null,"abstract":"<p >Depression, a prevalent mental illness, is intricately linked with the neurotransmitters in the brain, while serotonin as a crucial regulator of mood, energy levels, and memory, has been implicated in depression. So, the release of serotonin by serotonergic neurons plays a significant role in the development of depression. Notably, the foremost marker of oxidative stress, hydrogen peroxide (H<small><sub>2</sub></small>O<small><sub>2</sub></small>), can interfere with the functioning of serotonergic neurons and potentially contribute to depression. Investigating the impact of H<small><sub>2</sub></small>O<small><sub>2</sub></small> on serotonergic neurons could offer valuable insights into the mechanisms underlying depression. However, there have been no effective tools for selectively imaging H<small><sub>2</sub></small>O<small><sub>2</sub></small> in these neurons so far. To address this gap, we created a small molecular fluorescent probe, <strong>PF-H<small><sub>2</sub></small>O<small><sub>2</sub></small></strong>, designed specifically for imaging H<small><sub>2</sub></small>O<small><sub>2</sub></small> in serotonergic neurons under oxidative stress. <strong>PF-H<small><sub>2</sub></small>O<small><sub>2</sub></small></strong> exerts excellent serotonergic neuron-targetability and notable selectivity for H<small><sub>2</sub></small>O<small><sub>2</sub></small>. Furthermore, we discovered increased H<small><sub>2</sub></small>O<small><sub>2</sub></small> in serotonergic neurons of mice with depressive symptoms. Altogether, this endeavour unveils a pioneering tool for exploring pathophysiology linked to serotonergic neuronal dysfunction.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":null,"pages":null},"PeriodicalIF":6.1000,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Materials Chemistry B","FirstCategoryId":"1","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2024/tb/d4tb01828a","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
引用次数: 0
Abstract
Depression, a prevalent mental illness, is intricately linked with the neurotransmitters in the brain, while serotonin as a crucial regulator of mood, energy levels, and memory, has been implicated in depression. So, the release of serotonin by serotonergic neurons plays a significant role in the development of depression. Notably, the foremost marker of oxidative stress, hydrogen peroxide (H2O2), can interfere with the functioning of serotonergic neurons and potentially contribute to depression. Investigating the impact of H2O2 on serotonergic neurons could offer valuable insights into the mechanisms underlying depression. However, there have been no effective tools for selectively imaging H2O2 in these neurons so far. To address this gap, we created a small molecular fluorescent probe, PF-H2O2, designed specifically for imaging H2O2 in serotonergic neurons under oxidative stress. PF-H2O2 exerts excellent serotonergic neuron-targetability and notable selectivity for H2O2. Furthermore, we discovered increased H2O2 in serotonergic neurons of mice with depressive symptoms. Altogether, this endeavour unveils a pioneering tool for exploring pathophysiology linked to serotonergic neuronal dysfunction.
期刊介绍:
Journal of Materials Chemistry A, B & C cover high quality studies across all fields of materials chemistry. The journals focus on those theoretical or experimental studies that report new understanding, applications, properties and synthesis of materials. Journal of Materials Chemistry A, B & C are separated by the intended application of the material studied. Broadly, applications in energy and sustainability are of interest to Journal of Materials Chemistry A, applications in biology and medicine are of interest to Journal of Materials Chemistry B, and applications in optical, magnetic and electronic devices are of interest to Journal of Materials Chemistry C.Journal of Materials Chemistry B is a Transformative Journal and Plan S compliant. Example topic areas within the scope of Journal of Materials Chemistry B are listed below. This list is neither exhaustive nor exclusive:
Antifouling coatings
Biocompatible materials
Bioelectronics
Bioimaging
Biomimetics
Biomineralisation
Bionics
Biosensors
Diagnostics
Drug delivery
Gene delivery
Immunobiology
Nanomedicine
Regenerative medicine & Tissue engineering
Scaffolds
Soft robotics
Stem cells
Therapeutic devices