A versatile fluorescent probe for hydrogen peroxide in serotonergic neurons of living brains of mice with depression†

IF 6.1 3区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS
Feida Che, Xiaoming Zhao, Qi Ding, Xiwei Li, Wen Zhang, Ping Li, Xin Wang and Bo Tang
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引用次数: 0

Abstract

Depression, a prevalent mental illness, is intricately linked with the neurotransmitters in the brain, while serotonin as a crucial regulator of mood, energy levels, and memory, has been implicated in depression. So, the release of serotonin by serotonergic neurons plays a significant role in the development of depression. Notably, the foremost marker of oxidative stress, hydrogen peroxide (H2O2), can interfere with the functioning of serotonergic neurons and potentially contribute to depression. Investigating the impact of H2O2 on serotonergic neurons could offer valuable insights into the mechanisms underlying depression. However, there have been no effective tools for selectively imaging H2O2 in these neurons so far. To address this gap, we created a small molecular fluorescent probe, PF-H2O2, designed specifically for imaging H2O2 in serotonergic neurons under oxidative stress. PF-H2O2 exerts excellent serotonergic neuron-targetability and notable selectivity for H2O2. Furthermore, we discovered increased H2O2 in serotonergic neurons of mice with depressive symptoms. Altogether, this endeavour unveils a pioneering tool for exploring pathophysiology linked to serotonergic neuronal dysfunction.

Abstract Image

Abstract Image

抑郁症小鼠活体大脑血清素能神经元中过氧化氢的多功能荧光探针
抑郁症作为一种普遍的精神疾病,与大脑中的神经递质有着千丝万缕的联系,而血清素作为情绪、能量水平和记忆力的重要调节剂,与抑郁症有着千丝万缕的联系。因此,血清素能神经元释放的血清素在抑郁症的发病中起着重要作用。值得注意的是,氧化应激的首要标志物过氧化氢(H2O2)会干扰血清素能神经元的功能,并可能导致抑郁症。研究 H2O2 对血清素能神经元的影响可为了解抑郁症的发病机制提供宝贵的信息。然而,迄今为止还没有有效的工具对这些神经元中的 H2O2 进行选择性成像。为了填补这一空白,我们设计了一种小分子荧光探针 PF-H2O2,专门用于对氧化应激下血清素能神经元中的 H2O2 进行成像。PF-H2O2 具有出色的血清素能神经元靶向性和对 H2O2 的显著选择性。此外,我们还发现有抑郁症状的小鼠血清素能神经元中的 H2O2 增加了。总之,这项研究为探索与血清素能神经元功能障碍有关的病理生理学提供了一种开创性的工具。
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来源期刊
Journal of Materials Chemistry B
Journal of Materials Chemistry B MATERIALS SCIENCE, BIOMATERIALS-
CiteScore
11.50
自引率
4.30%
发文量
866
期刊介绍: Journal of Materials Chemistry A, B & C cover high quality studies across all fields of materials chemistry. The journals focus on those theoretical or experimental studies that report new understanding, applications, properties and synthesis of materials. Journal of Materials Chemistry A, B & C are separated by the intended application of the material studied. Broadly, applications in energy and sustainability are of interest to Journal of Materials Chemistry A, applications in biology and medicine are of interest to Journal of Materials Chemistry B, and applications in optical, magnetic and electronic devices are of interest to Journal of Materials Chemistry C.Journal of Materials Chemistry B is a Transformative Journal and Plan S compliant. Example topic areas within the scope of Journal of Materials Chemistry B are listed below. This list is neither exhaustive nor exclusive: Antifouling coatings Biocompatible materials Bioelectronics Bioimaging Biomimetics Biomineralisation Bionics Biosensors Diagnostics Drug delivery Gene delivery Immunobiology Nanomedicine Regenerative medicine & Tissue engineering Scaffolds Soft robotics Stem cells Therapeutic devices
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