Transcriptomic profiling of the floral fragrance biosynthesis of Iris germanica ‘Harvest of Memories’ and functional characterization of the IgTPS14 gene

IF 5.7 1区农林科学 Q1 HORTICULTURE

Horticultural Plant Journal Pub Date : 2024-09-14 DOI:10.1016/j.hpj.2024.06.004

Qian Zhao, Yuqing Li, Lina Gu, Di He, Jianrang Luo, Yanlong Zhang

{"title":"Transcriptomic profiling of the floral fragrance biosynthesis of Iris germanica ‘Harvest of Memories’ and functional characterization of the IgTPS14 gene","authors":"Qian Zhao, Yuqing Li, Lina Gu, Di He, Jianrang Luo, Yanlong Zhang","doi":"10.1016/j.hpj.2024.06.004","DOIUrl":null,"url":null,"abstract":"Iris (<ce:italic>Iris germanica</ce:italic>) is a very popular ornamental plant and is known for the precious spice irone produced from its roots. Many iris varieties can also release fragrances through their flowers. However, the composition of iris aroma and the molecular mechanism of its synthesis have not been reported. In this study, we analyzed the volatile floral compounds of <ce:italic>I. germanica</ce:italic> ‘Harvest of Memories’ at different stages and in different tissues through headspace solid-phase microextraction gas chromatography-mass spectrometry. A total of 36 volatile compounds were identified, and linalool was the dominant component. Transcriptome analysis showed that 35 differentially expressed genes and 218 differentially expressed transcription factors were positively correlated with linalool release. According to the results of qRT-qPCR, the expression level of the <ce:italic>IgTPS14</ce:italic> gene was consistent with the release trend of linalool, suggesting that <ce:italic>IgTPS14</ce:italic> may play a certain role in linalool synthesis. Phylogenetic analysis showed that the IgTPS14 protein belonged to the TPS-g subfamily. An <ce:italic>in vitro</ce:italic> enzymatic assay of <ce:italic>IgTPS14</ce:italic> and its transient and stable overexpression in tobacco indicated that this gene produced linalool. The transient silencing of <ce:italic>IgTPS14</ce:italic> in petals by virus-induced gene silencing technology revealed a significant reduction in the release of linalool. These results will help explore and reveal the molecular mechanism of monoterpenoid synthesis and provide a certain reference for studying the formation of iris aroma.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"9 1","pages":""},"PeriodicalIF":5.7000,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Horticultural Plant Journal","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1016/j.hpj.2024.06.004","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"HORTICULTURE","Score":null,"Total":0}

引用次数: 0

Abstract

Iris (Iris germanica) is a very popular ornamental plant and is known for the precious spice irone produced from its roots. Many iris varieties can also release fragrances through their flowers. However, the composition of iris aroma and the molecular mechanism of its synthesis have not been reported. In this study, we analyzed the volatile floral compounds of I. germanica ‘Harvest of Memories’ at different stages and in different tissues through headspace solid-phase microextraction gas chromatography-mass spectrometry. A total of 36 volatile compounds were identified, and linalool was the dominant component. Transcriptome analysis showed that 35 differentially expressed genes and 218 differentially expressed transcription factors were positively correlated with linalool release. According to the results of qRT-qPCR, the expression level of the IgTPS14 gene was consistent with the release trend of linalool, suggesting that IgTPS14 may play a certain role in linalool synthesis. Phylogenetic analysis showed that the IgTPS14 protein belonged to the TPS-g subfamily. An in vitro enzymatic assay of IgTPS14 and its transient and stable overexpression in tobacco indicated that this gene produced linalool. The transient silencing of IgTPS14 in petals by virus-induced gene silencing technology revealed a significant reduction in the release of linalool. These results will help explore and reveal the molecular mechanism of monoterpenoid synthesis and provide a certain reference for studying the formation of iris aroma.

查看原文本刊更多论文

德国鸢尾花'Harvest of Memories'花香生物合成的转录组分析和 IgTPS14 基因的功能特征描述

鸢尾花（Iris germanica）是一种非常受欢迎的观赏植物，因其根部产生的珍贵香料鸢尾酮而闻名。许多鸢尾品种也能通过花朵释放香气。然而，鸢尾花香气的成分及其合成的分子机制尚未见报道。在这项研究中，我们通过顶空固相微萃取气相色谱-质谱法分析了德国鸢尾花'Harvest of Memories'在不同阶段和不同组织中的挥发性花香化合物。共鉴定出 36 种挥发性化合物，其中芳樟醇是主要成分。转录组分析表明，35 个差异表达基因和 218 个差异表达转录因子与芳樟醇的释放呈正相关。根据 qRT-qPCR 的结果，IgTPS14 基因的表达水平与芳樟醇的释放趋势一致，表明 IgTPS14 可能在芳樟醇的合成过程中发挥了一定的作用。系统进化分析表明，IgTPS14 蛋白属于 TPS-g 亚家族。对 IgTPS14 的体外酶学检测及其在烟草中的瞬时稳定过表达表明，该基因能产生芳樟醇。通过病毒诱导的基因沉默技术对花瓣中的 IgTPS14 进行瞬时沉默，发现其释放的芳樟醇显著减少。这些结果将有助于探索和揭示单萜合成的分子机制，为研究鸢尾香气的形成提供一定的参考。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Horticultural Plant Journal Environmental Science-Ecology

CiteScore

9.60

自引率

14.00%

发文量

293

审稿时长

33 weeks

期刊介绍： Horticultural Plant Journal (HPJ) is an OPEN ACCESS international journal. HPJ publishes research related to all horticultural plants, including fruits, vegetables, ornamental plants, tea plants, and medicinal plants, etc. The journal covers all aspects of horticultural crop sciences, including germplasm resources, genetics and breeding, tillage and cultivation, physiology and biochemistry, ecology, genomics, biotechnology, plant protection, postharvest processing, etc. Article types include Original research papers, Reviews, and Short communications.