A highly specific red-emitting fluorescent probe for monitoring of Cu2+ in living cells and zebrafish

IF 3.2 4区化学 Q2 CHEMISTRY, ANALYTICAL

Luminescence Pub Date : 2024-09-16 DOI:10.1002/bio.4886

Shuai Kong, Yan Zhang, Lianke Xie, Guodong Qi, Yanping Li, Haitao Dai, Qiubo Qi, Yimin Chen

引用次数: 0

Abstract

A novel seminaphthorhodafluor-based fluorescent probe was designed and prepared. It exhibited an outstanding off–on fluorescence response (about 45-fold enhancement) to Cu²⁺ because the structure changed from a spirocyclic form to a ring-open state. It showed satisfactory detection performances for Cu²⁺ with fast response speed, high selectivity and anti-interference, and a low detection limit (22.9 nM) due to its long emission wavelength and off–on fluorescence response mode. This probe was also satisfactorily applied for the monitoring of Cu²⁺ in living cells and zebrafish.

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用于监测活细胞和斑马鱼体内 Cu2+ 的高特异性红色发光荧光探针

设计并制备了一种新型半萘基氟荧光探针。由于其结构从螺环状变为开环状，因此对 Cu2+ 的荧光响应非常显著（增强了约 45 倍）。该探针对 Cu2+ 的检测性能令人满意，具有响应速度快、选择性高、抗干扰性强等特点，而且由于其发射波长长和离体荧光响应模式，检测限低（22.9 nM）。该探针在活细胞和斑马鱼体内 Cu2+ 的监测方面也取得了令人满意的效果。

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来源期刊

Luminescence 生物-生化与分子生物学

CiteScore

5.10

自引率

13.80%

发文量

248

审稿时长

3.5 months

期刊介绍： Luminescence provides a forum for the publication of original scientific papers, short communications, technical notes and reviews on fundamental and applied aspects of all forms of luminescence, including bioluminescence, chemiluminescence, electrochemiluminescence, sonoluminescence, triboluminescence, fluorescence, time-resolved fluorescence and phosphorescence. Luminescence publishes papers on assays and analytical methods, instrumentation, mechanistic and synthetic studies, basic biology and chemistry. Luminescence also publishes details of forthcoming meetings, information on new products, and book reviews. A special feature of the Journal is surveys of the recent literature on selected topics in luminescence.