Ke-Le Chen, Dr. Ru-Jia Yu, Cheng-Bing Zhong, Ziyi Wang, Bao-Kang Xie, Dr. Hui Ma, Mingjun Ao, Prof. Peng Zheng, Prof. Andrew G. Ewing, Prof. Yi-Tao Long
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引用次数: 0
Abstract
A synapse-mimicking nanopipette method was developed to electrochemically monitor single vesicle dynamics induced by lipid-tau interactions. To mimic the lipid environment in synapses, model vesicles and a modified lipid layer were confined within a nanopipette, as reported by Ru-Jia Yu, Yi-Tao Long et al. in their Communication (e202406677). Characteristic ionic current oscillations were recorded and could be used to evaluate the binding affinity of tau protein, indicating that phosphorylated tau inhibits vesicle dynamics through strong interactions with synaptic vesicles.
我们开发了一种模拟突触的纳米吸管方法，用于电化学监测由脂质-Tau相互作用引起的单个囊泡动力学。为了模拟突触中的脂质环境，如 Ru-Jia Yu、Yi-Tao Long 等人在他们的通讯（e202406677）中所报道的那样，将模型囊泡和改性脂质层封闭在纳米吸管中。记录到的特征性离子电流振荡可用于评估tau蛋白的结合亲和力，表明磷酸化tau通过与突触小泡的强烈相互作用抑制了小泡的动力学。
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