Effects of ultrasonic degradation on physicochemical and antioxidant properties of Gleditsia sinensis seed polysaccharides

IF 2.4 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research Pub Date : 2024-09-12 DOI:10.1016/j.carres.2024.109272

Qiuping Yao , Longlin Pu , Boyu Dong , Dequan Zhu , Wenwen Wu , Qiong Yang

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Abstract

In this study, two degraded polysaccharides from Gleditsia sinensis seed were obtained under ultrasonic power treatments of 300 and 450 W. The physicochemical properties, structural characteristics, and antioxidant activities of the degraded and undegraded polysaccharides were studied and compared. Ion exchange chromatography and methylation analysis showed that the polysaccharides had similar basic structural features and were composed of the same monosaccharide units before and after degradation, but the ultrasonic treatment increased the total monosaccharide content and changed the Mannose/Galactose value. Furthermore, with the increase in the ultrasonic power, the molecular weight and intrinsic viscosity of polysaccharides decreased, and the micromorphology became looser. The scavenging capacities for 1,1-diphenyl-2-picrylhydrazyl and hydroxyl free radicals and the reducing ability were significantly increased by the ultrasonic treatment. In conclusion, ultrasonic treatment may be an effective way to improve the antioxidant activities of polysaccharides from G. sinensis seed, and further studies on its antioxidant mechanism are still needed.

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超声降解对皂荚多糖理化性质和抗氧化性的影响

研究比较了降解多糖和未降解多糖的理化性质、结构特征和抗氧化活性。离子交换色谱法和甲基化分析表明，降解前后的多糖具有相似的基本结构特征，由相同的单糖单元组成，但超声处理增加了总单糖含量，改变了甘露糖/半乳糖值。此外，随着超声功率的增加，多糖的分子量和固有粘度降低，微观形态变得疏松。超声波处理对 1,1-二苯基-2-苦基肼和羟基自由基的清除能力和还原能力显著提高。总之，超声波处理可能是提高冬凌草种子多糖抗氧化活性的一种有效方法，但其抗氧化机理仍有待进一步研究。

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来源期刊

Carbohydrate Research 化学-生化与分子生物学

CiteScore

5.00

自引率

3.20%

发文量

183

审稿时长

3.6 weeks

期刊介绍： Carbohydrate Research publishes reports of original research in the following areas of carbohydrate science: action of enzymes, analytical chemistry, biochemistry (biosynthesis, degradation, structural and functional biochemistry, conformation, molecular recognition, enzyme mechanisms, carbohydrate-processing enzymes, including glycosidases and glycosyltransferases), chemical synthesis, isolation of natural products, physicochemical studies, reactions and their mechanisms, the study of structures and stereochemistry, and technological aspects. Papers on polysaccharides should have a "molecular" component; that is a paper on new or modified polysaccharides should include structural information and characterization in addition to the usual studies of rheological properties and the like. A paper on a new, naturally occurring polysaccharide should include structural information, defining monosaccharide components and linkage sequence. Papers devoted wholly or partly to X-ray crystallographic studies, or to computational aspects (molecular mechanics or molecular orbital calculations, simulations via molecular dynamics), will be considered if they meet certain criteria. For computational papers the requirements are that the methods used be specified in sufficient detail to permit replication of the results, and that the conclusions be shown to have relevance to experimental observations - the authors'' own data or data from the literature. Specific directions for the presentation of X-ray data are given below under Results and "discussion".