Design and optimisation of lysozyme protein purification process using non-thermal progressive freeze concentration technology

IF 3.8 3区工程技术 Q3 ENERGY & FUELS

Chemical Engineering and Processing - Process Intensification Pub Date : 2024-09-06 DOI:10.1016/j.cep.2024.109975

Tazien Rashid , Mazura Jusoh , Zaki Yamani Zakaria , Norshafika Yahya , Sabah Ansar , Tiong Sieh Kiong , Farooq Sher

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引用次数: 0

Abstract

In this study, PFC system is investigated to improve the concentration and yield of lysozyme. The research focused on an attempt to thoroughly construct an ice crystallizer with measurable and optimized design parameters for an efficient lysozyme protein concentration procedure because the productivity of PFC is always an issue. A new Multiple Probe Cryo-Concentrator (MPCC) device was designed and successfully equipped with probes having a well-distributed cooled surface area for ice crystallization with proper internal cooling temperature control as well as a solution movement mechanism provided by a stirrer in the tank. The impact of different operating parameters is optimally investigated. Central Composite Design (CCD) is utilized to optimize PFC operating conditions and their response to partition constant (K-value) and solute yield. The results showed that a coolant temperature of -12 ⁰C, stirrer speed of 350 rpm, operation time of 40 min and initial concentration of 10 mg/mL gave the best K-value (0.132) and solute concentration yield (87.39 %). The design elements of the equipment are crucial in providing improved PFC performance. The study revealed that the PFC system designed and applied in this study can improve the lysozyme protein concentration as needed in the food and pharmaceutical industry.

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利用非热渐进式冷冻浓缩技术设计和优化溶菌酶蛋白纯化工艺

本研究调查了 PFC 系统，以提高溶菌酶的浓度和产量。由于 PFC 的生产率一直是个问题，因此研究重点是尝试彻底构建一个具有可测量和优化设计参数的冰结晶器，以实现高效的溶菌酶蛋白浓缩过程。研究人员设计了一种新型多探针低温浓缩器（MPCC）装置，并成功配备了具有良好冷却表面积的探针，用于适当控制内部冷却温度的冰结晶，以及由槽内搅拌器提供的溶液运动机制。对不同操作参数的影响进行了优化研究。利用中央复合设计（CCD）来优化 PFC 运行条件及其对分配常数（K 值）和溶质产量的影响。结果表明，冷却剂温度为 -12 ⁰C、搅拌器转速为 350 rpm、操作时间为 40 分钟、初始浓度为 10 mg/mL，可获得最佳 K 值（0.132）和溶质浓度产率（87.39%）。设备的设计要素对提高全氟化学品的性能至关重要。研究表明，本研究中设计和应用的 PFC 系统可以提高食品和制药行业所需的溶菌酶蛋白浓度。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemical Engineering and Processing - Process Intensification 工程技术-工程：化工

CiteScore

7.80

自引率

9.30%

发文量

408

审稿时长

49 days

期刊介绍： Chemical Engineering and Processing: Process Intensification is intended for practicing researchers in industry and academia, working in the field of Process Engineering and related to the subject of Process Intensification.Articles published in the Journal demonstrate how novel discoveries, developments and theories in the field of Process Engineering and in particular Process Intensification may be used for analysis and design of innovative equipment and processing methods with substantially improved sustainability, efficiency and environmental performance.