Droplet-based single-cell sequencing: Strategies and applications

IF 12.1 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances Pub Date : 2024-09-11 DOI:10.1016/j.biotechadv.2024.108454

Yuting Shang , Zhengzheng Wang , Liqing Xi , Yantao Wang , Meijing Liu , Ying Feng , Juan Wang , Qingping Wu , Xinran Xiang , Moutong Chen , Yu Ding

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引用次数: 0

Abstract

Notable advancements in single-cell omics technologies have not only addressed longstanding challenges but also enabled unprecedented studies of cellular heterogeneity with unprecedented resolution and scale. These strides have led to groundbreaking insights into complex biological systems, paving the way for a more profound comprehension of human biology and diseases. The droplet microfluidic technology has become a crucial component in many single-cell sequencing workflows in terms of throughput, cost-effectiveness, and automation. Utilizing a microfluidic chip to encapsulate and profile individual cells within droplets has significantly improved single-cell research. Therefore, this review aims to comprehensively elaborate the droplet microfluidics-assisted omics methods from a single-cell perspective. The strategies for using droplet microfluidics in the realms of genomics, epigenomics, transcriptomics, and proteomics analyses are first introduced. On this basis, the focus then turns to the latest applications of this technology in different sequencing patterns, including mono- and multi-omics. Finally, the challenges and further perspectives of droplet-based single-cell sequencing in both foundational research and commercial applications are discussed.

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基于液滴的单细胞测序：策略与应用

单细胞奥米克斯技术的显著进步不仅解决了长期存在的难题，还以前所未有的分辨率和规模对细胞异质性进行了前所未有的研究。这些进步使人们对复杂的生物系统有了突破性的认识，为更深入地了解人类生物学和疾病铺平了道路。液滴微流控技术在通量、成本效益和自动化方面已成为许多单细胞测序工作流程的重要组成部分。利用微流控芯片将单个细胞封装在液滴中并对其进行分析，大大提高了单细胞研究的效率。因此，本综述旨在从单细胞角度全面阐述液滴微流控辅助的omics方法。首先介绍了在基因组学、表观基因组学、转录组学和蛋白质组学分析领域使用液滴微流控技术的策略。在此基础上，重点转向该技术在不同测序模式中的最新应用，包括单组学和多组学。最后，讨论了基于液滴的单细胞测序技术在基础研究和商业应用中面临的挑战和进一步的发展前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnology advances 工程技术-生物工程与应用微生物

CiteScore

25.50

自引率

2.50%

发文量

167

审稿时长

37 days

期刊介绍： Biotechnology Advances is a comprehensive review journal that covers all aspects of the multidisciplinary field of biotechnology. The journal focuses on biotechnology principles and their applications in various industries, agriculture, medicine, environmental concerns, and regulatory issues. It publishes authoritative articles that highlight current developments and future trends in the field of biotechnology. The journal invites submissions of manuscripts that are relevant and appropriate. It targets a wide audience, including scientists, engineers, students, instructors, researchers, practitioners, managers, governments, and other stakeholders in the field. Additionally, special issues are published based on selected presentations from recent relevant conferences in collaboration with the organizations hosting those conferences.