Effect of repeated low doses of gonadotropin-releasing hormone on the secretion of luteinizing hormone and follicle-stimulating hormone, and ovarian function in dairy cows suffering from anovulation type I

IF 2.2 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science Pub Date : 2024-09-16 DOI:10.1016/j.anireprosci.2024.107602

Wojciech Barański , Arkadiusz Nowicki , Mark A. Crowe , Dawid Tobolski , Sławomir Zduńczyk

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Abstract

The aim of the study was to evaluate the effect of repeated low doses of gonadotropin-releasing hormone (GnRH) agonist buserelin once a day for 5 days on follicle-stimulating hormone (FSH) and luteinizing hormone (LH) release, and ovarian function in dairy cows with anovulation type I. The study was conducted on 10 anovulatory Polish Holstein-Friesian cows. Cows in Group 1 (n = 5) received 4 µg of buserelin intramuscularly. once a day for 5 days. Control cows from Group 2 (n = 5) received saline. Concentrations of progesterone, FSH, and LH in the blood were analysed using radioimmunoassay (RIA). Ovarian structures were monitored weekly after the end of treatment by ultrasound for 4 weeks. Injections of buserelin increased FSH and LH concentrations. Release of FSH and LH was largely variable, but there was significant (P < 0.001) interaction between time and treatment during all treatment days. The number of follicles in the treatment group was significantly greater (P = 0.029) than in the control group on day 5, the diameter of follicles on day 12 was also larger (P < 0.01) in treated than in control cows. Ovulation occurred in 4 of the 5 treated cows, but only in 1 of the control cows in the 40 days after treatment. In conclusion, repeated low doses of GnRH analogue buserelin once a day for 5 days may increase the concentration of FSH and LH in dairy cows with anovulation type I.

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重复低剂量促性腺激素释放激素对I型无排卵奶牛黄体生成素和卵泡刺激素分泌及卵巢功能的影响

该研究的目的是评估每天一次、连续5天重复低剂量促性腺激素释放激素（GnRH）激动剂布舍瑞林对I型无排卵奶牛卵泡刺激素（FSH）和黄体生成素（LH）释放以及卵巢功能的影响。第 1 组奶牛（5 头）肌肉注射 4 µg 布舍瑞林，每天一次，连续 5 天。对照组 2 的奶牛（n = 5 头）接受生理盐水治疗。使用放射免疫分析法（RIA）分析血液中孕酮、FSH 和 LH 的浓度。治疗结束后，每周通过超声波监测卵巢结构，为期 4 周。注射丁螺环酮可增加 FSH 和 LH 的浓度。FSH和LH的释放在很大程度上是可变的，但在所有治疗日中，时间与治疗之间存在显著的交互作用（P < 0.001）。第5天，治疗组的卵泡数量明显多于对照组（P = 0.029），第12天，治疗组的卵泡直径也大于对照组（P < 0.01）。在治疗后的 40 天内，5 头治疗组奶牛中有 4 头排卵，而对照组奶牛中只有 1 头排卵。总之，每天一次、连续5天重复小剂量GnRH类似物布舍瑞林可提高I型无排卵奶牛的FSH和LH浓度。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Animal Reproduction Science 农林科学-奶制品与动物科学

CiteScore

4.50

自引率

9.10%

发文量

136

审稿时长

54 days

期刊介绍： Animal Reproduction Science publishes results from studies relating to reproduction and fertility in animals. This includes both fundamental research and applied studies, including management practices that increase our understanding of the biology and manipulation of reproduction. Manuscripts should go into depth in the mechanisms involved in the research reported, rather than a give a mere description of findings. The focus is on animals that are useful to humans including food- and fibre-producing; companion/recreational; captive; and endangered species including zoo animals, but excluding laboratory animals unless the results of the study provide new information that impacts the basic understanding of the biology or manipulation of reproduction. The journal''s scope includes the study of reproductive physiology and endocrinology, reproductive cycles, natural and artificial control of reproduction, preservation and use of gametes and embryos, pregnancy and parturition, infertility and sterility, diagnostic and therapeutic techniques. The Editorial Board of Animal Reproduction Science has decided not to publish papers in which there is an exclusive examination of the in vitro development of oocytes and embryos; however, there will be consideration of papers that include in vitro studies where the source of the oocytes and/or development of the embryos beyond the blastocyst stage is part of the experimental design.